PMID- 18815023
OWN - NLM
STAT- MEDLINE
DCOM- 20090212
LR  - 20090714
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 24
IP  - 4
DP  - 2008 Dec 1
TI  - Rapid and separation-free sandwich immunosensing based on accumulation of 
      microbeads by negative-dielectrophoresis.
PG  - 1006-11
LID - 10.1016/j.bios.2008.08.002 [doi]
AB  - We report here a rapid and separation-free immunoassay using a dielectrophoresis 
      (DEP) device consisting of an interdigitated microarray (IDA) electrode and a 
      polydimethylsiloxane (PDMS) substrate. On applying an AC voltage to the IDA in a 
      negative-DEP (n-DEP) frequency region, goat anti-mouse immunoglobulin (anti-mouse 
      IgG)-immobilized microbeads moved to the surface of the PDMS substrate placed 
      above the IDA. The microbeads accumulated at designated areas of the PDMS surface 
      that had been precoated with anti-mouse IgG. When the fluorescence microbeads 
      bearing anti-mouse IgG were suspended in an analyte (mouse IgG) solution, the 
      microbeads trapped the analyte to form immunocomplexes on microbeads. The 
      microbeads reacted with mouse IgG accumulated and were captured at the designated 
      areas of the PDMS surface via an antibody-antigen-antibody (sandwich) reaction. 
      The captured microbeads were detected selectively by fluorescence measurements at 
      the focused designated areas, regardless of the presence of uncaptured microbeads 
      suspended in solution. Thus, the separation and washing-out steps usually 
      required for conventional immunoassay are eliminated in the presented procedure. 
      Since the formation of the sandwich structures was accelerated significantly by 
      n-DEP, a period as short as 30s was sufficient to detect the immunoreaction at 
      the surface. The fluorescence intensity of the captured microbeads at the 
      designated areas increased with analyte concentration in the range 0.01-10ng/mL. 
      The present procedure therefore yields a rapid, sensitive, and separation-free 
      immunoassay in a simple device.
FAU - Lee, Hyun Jung
AU  - Lee HJ
AD  - Graduate School of Environmental Studies, Tohoku University, 6-6-11-604, 
      Aramaki-Aoba, Aoba-Ku, Sendai 980-8579, Japan.
FAU - Yasukawa, Tomoyuki
AU  - Yasukawa T
FAU - Shiku, Hitoshi
AU  - Shiku H
FAU - Matsue, Tomokazu
AU  - Matsue T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080809
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Animals
MH  - Biosensing Techniques/*instrumentation/methods
MH  - Electrophoresis/*instrumentation
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Goats
MH  - Immunoassay/*instrumentation
MH  - Immunoglobulin G/*analysis/immunology
MH  - Mice
MH  - Microspheres
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Fluorescence/*instrumentation
EDAT- 2008/09/26 09:00
MHDA- 2009/02/13 09:00
CRDT- 2008/09/26 09:00
PHST- 2008/05/15 00:00 [received]
PHST- 2008/07/11 00:00 [revised]
PHST- 2008/08/04 00:00 [accepted]
PHST- 2008/09/26 09:00 [pubmed]
PHST- 2009/02/13 09:00 [medline]
PHST- 2008/09/26 09:00 [entrez]
AID - S0956-5663(08)00443-0 [pii]
AID - 10.1016/j.bios.2008.08.002 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2008 Dec 1;24(4):1006-11. doi: 10.1016/j.bios.2008.08.002. 
      Epub 2008 Aug 9.