PMID- 18815218
OWN - NLM
STAT- MEDLINE
DCOM- 20090312
LR  - 20190104
IS  - 1931-857X (Print)
IS  - 1522-1466 (Electronic)
IS  - 1522-1466 (Linking)
VI  - 295
IP  - 6
DP  - 2008 Dec
TI  - JAK2/Y343/STAT5 signaling axis is required for erythropoietin-mediated protection 
      against ischemic injury in primary renal tubular epithelial cells.
PG  - F1689-95
LID - 10.1152/ajprenal.90333.2008 [doi]
AB  - Erythropoietin has emerged as a potential therapy for the treatment of ischemic 
      tissue injury. In erythroid cells, the JAK2/Y343/STAT5 signaling axis has been 
      shown to be necessary for stress but not steady-state erythropoiesis. The 
      requirement for STAT5 activation in erythropoietin-mediated protection from 
      ischemic injury has not been well-studied. To answer this question, we induced 
      reproducible necrotic ischemic injury in primary mouse renal tubular epithelial 
      cells (RTEC) in vitro. Using RTEC from erythropoietin receptor mutant mice with 
      differential STAT5 signaling capabilities, we demonstrated first, that EPO 
      administration either before or during injury significantly protects against 
      mild-moderate but not severe necrotic cell death; and second, the JAK2/Y343/STAT5 
      signaling axis is required for protection against ischemic injury in primary 
      mouse RTEC. In addition, we identified Pim-3, a prosurvival STAT5 target gene, as 
      responsive to EPO in the noninjured kidney both in vitro and in vivo.
FAU - Breggia, A C
AU  - Breggia AC
AD  - Division of Nephrology and Transplantation, Maine Medical Center, 22 Bramhall 
      St., Portland, ME 04102, USA.
FAU - Wojchowski, D M
AU  - Wojchowski DM
FAU - Himmelfarb, J
AU  - Himmelfarb J
LA  - eng
GR  - P20 RR018789/RR/NCRR NIH HHS/United States
PT  - Journal Article
DEP - 20080924
PL  - United States
TA  - Am J Physiol Renal Physiol
JT  - American journal of physiology. Renal physiology
JID - 100901990
RN  - 0 (Actins)
RN  - 0 (STAT5 Transcription Factor)
RN  - 11096-26-7 (Erythropoietin)
RN  - EC 2.7.10.2 (Jak2 protein, mouse)
RN  - EC 2.7.10.2 (Janus Kinase 2)
SB  - IM
MH  - Actins/genetics
MH  - Animals
MH  - Cell Culture Techniques
MH  - Epithelial Cells/drug effects/*physiology
MH  - Erythropoietin/*pharmacology
MH  - Ischemia/*prevention & control
MH  - Janus Kinase 2/antagonists & inhibitors/*physiology
MH  - Kidney Tubules/cytology/drug effects/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - STAT5 Transcription Factor/*physiology
MH  - Signal Transduction/*physiology
PMC - PMC2753302
EDAT- 2008/09/26 09:00
MHDA- 2009/03/13 09:00
PMCR- 2008/09/24
CRDT- 2008/09/26 09:00
PHST- 2008/09/26 09:00 [pubmed]
PHST- 2009/03/13 09:00 [medline]
PHST- 2008/09/26 09:00 [entrez]
PHST- 2008/09/24 00:00 [pmc-release]
AID - 90333.2008 [pii]
AID - F-90333-2008 [pii]
AID - 10.1152/ajprenal.90333.2008 [doi]
PST - ppublish
SO  - Am J Physiol Renal Physiol. 2008 Dec;295(6):F1689-95. doi: 
      10.1152/ajprenal.90333.2008. Epub 2008 Sep 24.