PMID- 18817542
OWN - NLM
STAT- MEDLINE
DCOM- 20081126
LR  - 20231213
IS  - 1471-2172 (Electronic)
IS  - 1471-2172 (Linking)
VI  - 9
DP  - 2008 Sep 25
TI  - Direct contact of platelets and their released products exert different effects 
      on human dendritic cell maturation.
PG  - 54
LID - 10.1186/1471-2172-9-54 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are antigen presenting cells capable of 
      inducing innate and adaptive immune responses. According to the stimulus and 
      their maturation state, DCs induce immunogenic or tolerogenic responses. 
      Platelets (PLTs), which are involved in haemostasis and inflammation, can also 
      interact with DCs. In this study, we examined the effect of PLTs on DC maturation 
      in vitro. Human monocyte-derived DCs were co-cultured for 2 days with homologous 
      PLTs either in the same well or in 0.4 mum-pore size filter-separated 
      compartments. RESULTS: Confocal microscopy showed the attachment of PLTs to DC 
      membranes. The DC receptor involved in this interactions was found to be CD162. 
      In addition, we observed that DCs co-cultured with PLTs in filter-separated 
      compartments acquired a mature phenotype (high CD80, CD86, and intermediate CD83 
      expression; IL-12(p70) production; efficient stimulation of autologous CD4+ T 
      cell proliferation), while DCs co-cultured with PLTs in the same compartment did 
      not undergo phenotypic maturation, did not secrete IL-12(p70) or IL-1beta, but 
      instead induced moderate Th2-polarized T cell proliferation. CONCLUSION: These 
      data indicate that (i) PLTs secrete a soluble DC-activating factor that was 
      demonstrated not to be soluble CD40-Ligand (CD154; as could have been expected 
      from in vivo and previous in vitro work) but to be nucleotide, and (ii) that 
      cell-to-cell contact did not induce DC maturation, possibly because nucleotide 
      release by PLTs was prevented by direct contact with DCs. This work demonstrates 
      that PLTs are active elements of the immune system that might play a role in 
      balancing the ability of DCs to polarize T cell responses, therefore making them 
      critical factors in transfusion processes.
FAU - Hamzeh-Cognasse, Hind
AU  - Hamzeh-Cognasse H
AD  - Faculty of Medicine, Mucosal Immunity and Pathogen Agents Group, Jean Monnet 
      University of Saint-Etienne, Saint-Etienne, France. 
      hind.hamzeh@univ-st-etienne.fr
FAU - Cognasse, Fabrice
AU  - Cognasse F
FAU - Palle, Sabine
AU  - Palle S
FAU - Chavarin, Patricia
AU  - Chavarin P
FAU - Olivier, Thomas
AU  - Olivier T
FAU - Delezay, Olivier
AU  - Delezay O
FAU - Pozzetto, Bruno
AU  - Pozzetto B
FAU - Garraud, Olivier
AU  - Garraud O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080925
PL  - England
TA  - BMC Immunol
JT  - BMC immunology
JID - 100966980
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (P-selectin ligand protein)
SB  - IM
MH  - Antigens, CD/immunology/metabolism
MH  - B7-1 Antigen/immunology/metabolism
MH  - B7-2 Antigen/immunology/metabolism
MH  - Blood Platelets/cytology/*metabolism
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Differentiation
MH  - Coculture Techniques
MH  - Cytokines/immunology/metabolism
MH  - Dendritic Cells/cytology/*immunology/*metabolism
MH  - Humans
MH  - Immunoglobulins/immunology/metabolism
MH  - Lymphocyte Activation
MH  - Membrane Glycoproteins/immunology/metabolism
MH  - CD83 Antigen
PMC - PMC2564901
EDAT- 2008/09/27 09:00
MHDA- 2008/12/17 09:00
PMCR- 2008/09/25
CRDT- 2008/09/27 09:00
PHST- 2008/03/10 00:00 [received]
PHST- 2008/09/25 00:00 [accepted]
PHST- 2008/09/27 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/09/27 09:00 [entrez]
PHST- 2008/09/25 00:00 [pmc-release]
AID - 1471-2172-9-54 [pii]
AID - 10.1186/1471-2172-9-54 [doi]
PST - epublish
SO  - BMC Immunol. 2008 Sep 25;9:54. doi: 10.1186/1471-2172-9-54.