PMID- 18820821
OWN - NLM
STAT- MEDLINE
DCOM- 20081230
LR  - 20190911
IS  - 0355-3140 (Print)
IS  - 0355-3140 (Linking)
VI  - 34
IP  - 4
DP  - 2008 Aug
TI  - Inflammation but no DNA (deoxyribonucleic acid) damage in mice exposed to 
      airborne dust from a biofuel plant.
PG  - 278-7
AB  - OBJECTIVES: Particles in ambient air are associated with such health effects as 
      lung diseases and cancer of the lung. Exposure to bioaerosols has been found to 
      be associated with respiratory symptoms. The toxic properties of exposure to 
      combustion and bioaerosol particles from biofuel plants have not been studied in 
      detail. This study investigated whether exposure to dust from biofuel plants 
      induces DNA (deoxyribonucleic acid) damage and inflammation in exposed mice. 
      METHODS: DNA damage and inflammation were evaluated in mice exposed through the 
      intratracheal installation of airborne dust collected at a biofuel plant at the 
      straw storage hall and in the boiler room. The mice were given either a single 
      dose of dust (18 or 54 microg) or four doses of 54 microg on each of four 
      consecutive days. Control mice were exposed to a 0.9% sodium chloride solution. 
      RESULTS: In the mice exposed to 4 x 54 microg of dust, the lung tissue mRNA 
      (messenger ribonucleic acid) levels of interleukin-6 (IL-6), monocyte 
      chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein-2 (MIP-2) 
      were increased more than 10-fold if the dust was from the boiler room and 30- to 
      60-fold if the dust came from the straw storage hall. The levels of DNA strand 
      breaks in broncheoalveolar lavage (BAL) cells from the mice exposed to dust did 
      not differ from those in the control samples. CONCLUSIONS: The results indicate 
      that the instillation of dust from a biofuel plant, at doses corresponding to 2 
      weeks of exposure to human endotoxins, results in a strong inflammatory response 
      without detectable DNA damage in BAL cells. The dust from the straw storage hall 
      induced the strongest inflammatory response and had the highest concentration of 
      most microbial components.
FAU - Madsen, Anne Mette
AU  - Madsen AM
AD  - National Research Centre for the Working Environment, Lerso Parkalle 105, 
      Copenhagen, Denmark. amm@nrcwe.dk
FAU - Saber, Anne Thoustrup
AU  - Saber AT
FAU - Nordly, Pernille
AU  - Nordly P
FAU - Sharma, Anoop Kumar
AU  - Sharma AK
FAU - Wallin, Hakan
AU  - Wallin H
FAU - Vogel, Ulla
AU  - Vogel U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Finland
TA  - Scand J Work Environ Health
JT  - Scandinavian journal of work, environment & health
JID - 7511540
RN  - 0 (Aerosols)
RN  - 0 (Air Pollutants, Occupational)
RN  - 0 (Dust)
RN  - 0 (Endotoxins)
SB  - IM
MH  - Aerosols
MH  - Air Pollutants, Occupational/adverse effects/*analysis
MH  - Animals
MH  - *Bioelectric Energy Sources
MH  - Colony Count, Microbial
MH  - DNA Damage
MH  - Denmark
MH  - Dust/*analysis
MH  - Endotoxins/analysis
MH  - Humans
MH  - Inflammation/etiology
MH  - Lung/pathology
MH  - Mice
MH  - Occupational Exposure/adverse effects/*analysis
MH  - Respiratory Tract Diseases/*prevention & control
EDAT- 2008/09/30 09:00
MHDA- 2008/12/31 09:00
CRDT- 2008/09/30 09:00
PHST- 2008/09/30 09:00 [pubmed]
PHST- 2008/12/31 09:00 [medline]
PHST- 2008/09/30 09:00 [entrez]
AID - 1272 [pii]
AID - 10.5271/sjweh.1272 [doi]
PST - ppublish
SO  - Scand J Work Environ Health. 2008 Aug;34(4):278-7. doi: 10.5271/sjweh.1272.