PMID- 18824727
OWN - NLM
STAT- MEDLINE
DCOM- 20090206
LR  - 20220316
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 50
IP  - 2
DP  - 2009 Feb
TI  - Chromosome 3 intratumor heterogeneity in uveal melanoma.
PG  - 500-4
LID - 10.1167/iovs.08-2279 [doi]
AB  - PURPOSE: To investigate the presence of focal or diffuse heterogeneity of 
      monosomy 3 in uveal melanoma, by using fluorescence in situ hybridization (FISH). 
      METHODS: Direct interphase FISH in a series of 151 uveal melanomas revealed 82 
      tumors with loss of chromosome 3. Tumors with monosomy 3 were suspected to be 
      heterogeneous if there were low percentages of monosomy 3, triploid clones, 
      inconsistencies between FISH on centromere 3 and the long arm of chromosome 3, or 
      discrepancies between fine-needle-aspiration biopsies (FNABs) and the main tumor. 
      These tumors (n=16), all choroidal melanomas, were selected and analyzed for 
      intratumor heterogeneity by using FISH on paraffin-embedded tissue sections. 
      RESULTS: Different sections of each tumor were evaluated with FISH: 6 tumors 
      showed monosomy 3 in the same percentage throughout the tumor, and 10 showed 
      multiple clones with different percentages of monosomy 3. However, these tumors 
      did not show focal heterogeneity with respect to chromosome 3 status, and 
      differences in monosomy 3 distribution between the base and apex of the tumor 
      could not be identified. CONCLUSIONS: Although a small number of uveal melanomas 
      show heterogeneity for chromosome 3, it does not affect survival. In the presence 
      of triploid clones, the loss of chromosome 3 is more difficult to interpret. In 
      general, tumor biopsies in uveal melanoma provide an accurate prediction of the 
      patient's prognosis.
FAU - Mensink, Hanneke W
AU  - Mensink HW
AD  - The Rotterdam Eye Hospital, Rotterdam, The Netherlands.
FAU - Vaarwater, Jolanda
AU  - Vaarwater J
FAU - Kilic, Emine
AU  - Kilic E
FAU - Naus, Nicole C
AU  - Naus NC
FAU - Mooy, Neeltje
AU  - Mooy N
FAU - Luyten, Gre
AU  - Luyten G
FAU - Bruggenwirth, Hennie T
AU  - Bruggenwirth HT
FAU - Paridaens, Dion
AU  - Paridaens D
FAU - de Klein, Annelies
AU  - de Klein A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080929
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Choroid Neoplasms/*genetics/pathology
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Female
MH  - *Genetic Heterogeneity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*genetics/pathology
MH  - Middle Aged
MH  - Monosomy/*genetics/pathology
MH  - Prognosis
EDAT- 2008/10/01 09:00
MHDA- 2009/02/07 09:00
CRDT- 2008/10/01 09:00
PHST- 2008/10/01 09:00 [pubmed]
PHST- 2009/02/07 09:00 [medline]
PHST- 2008/10/01 09:00 [entrez]
AID - iovs.08-2279 [pii]
AID - 10.1167/iovs.08-2279 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2009 Feb;50(2):500-4. doi: 10.1167/iovs.08-2279. Epub 
      2008 Sep 29.