PMID- 18829400
OWN - NLM
STAT- MEDLINE
DCOM- 20081203
LR  - 20131121
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 874
IP  - 1-2
DP  - 2008 Oct 15
TI  - Liquid chromatographic-electrospray ionization mass spectrometric assay for 
      simultaneous determination of 3,4-methylenedioxymethamphetamine and its 
      metabolites 3,4-methylenedioxyamphetamine, 3,4-dihydroxymethamphetamine, and 
      4-hydroxy-3-methoxymethamphetamine in rat brain.
PG  - 119-24
LID - 10.1016/j.jchromb.2008.09.009 [doi]
AB  - 3,4-Methylenedioxymethamphetamine (MDMA) is a psychoactive drug with abuse 
      liability and neurotoxic potential. Mechanisms by which MDMA produces behavioral 
      and neurotoxic effects have yet to be elucidated. By measuring concentrations of 
      MDMA and its metabolites in relevant brain sites, it may be possible to gain 
      insight into mechanisms underlying MDMA actions. For this purpose, an LC-MS assay 
      with electrospray ionization was developed after homogenization of rat brain and 
      enzymatic conjugate cleavage. The method was successfully validated with respect 
      to selectivity, linearity, accuracy, precision, recovery, and matrix effect and 
      its use should help to delineate the neurotoxic mechanism of action of MDMA.
FAU - Mueller, Melanie
AU  - Mueller M
AD  - Department of Experimental and Clinical Toxicology, Institute of Experimental and 
      Clinical Pharmacology and Toxicology, Saarland University, D-66421 Homburg 
      (Saar), Germany.
FAU - Peters, Frank T
AU  - Peters FT
FAU - Ricaurte, George A
AU  - Ricaurte GA
FAU - Maurer, Hans H
AU  - Maurer HH
LA  - eng
GR  - DA017964/DA/NIDA NIH HHS/United States
GR  - DA05707/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080917
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 117652-28-5 (4-hydroxy-3-methoxymethamphetamine)
RN  - 15398-87-5 (alpha-methylepinine)
RN  - 44RAL3456C (Methamphetamine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - R7339QLN1C (Deoxyepinephrine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*analysis/metabolism
MH  - Animals
MH  - Brain/metabolism
MH  - Brain Chemistry
MH  - Chromatography, Liquid/*methods
MH  - Deoxyepinephrine/*analogs & derivatives/analysis/metabolism
MH  - Methamphetamine/*analogs & derivatives/analysis/metabolism
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*analysis/metabolism
MH  - Rats
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2008/10/03 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/10/03 09:00
PHST- 2008/07/03 00:00 [received]
PHST- 2008/09/02 00:00 [revised]
PHST- 2008/09/03 00:00 [accepted]
PHST- 2008/10/03 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/10/03 09:00 [entrez]
AID - S1570-0232(08)00665-X [pii]
AID - 10.1016/j.jchromb.2008.09.009 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Oct 15;874(1-2):119-24. doi: 
      10.1016/j.jchromb.2008.09.009. Epub 2008 Sep 17.