PMID- 18835233
OWN - NLM
STAT- MEDLINE
DCOM- 20081204
LR  - 20131121
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 383
IP  - 2
DP  - 2008 Dec 15
TI  - A comparison between the responses of neutral red and acridine orange: acridine 
      orange should be preferential and alternative to neutral red as a dye for the 
      monitoring of contaminants by means of biological sensors.
PG  - 316-9
LID - 10.1016/j.ab.2008.09.015 [doi]
AB  - The acridine orange (AO) and neutral red (NR) dyes, commonly used as probes to 
      measure the internal pH in acidic vesicles, are compared in this article. The 
      comparison between the two dyes (arising from calculations taking into account 
      their analytical constants) illustrated that the use of AO is preferential to 
      that of NR because the AO response is sensitive over the whole pH range between 
      4.0 and 7.4, whereas the NR response is effective only between pHs 4.0 and 6.0. 
      In addition, it became evident from the mitochondrial respiration response that 
      NR, unlike AO, is a protonophore. When taken into consideration, these two 
      properties suggest that AO is more suitable than NR as an indicator of toxicity 
      measurements in water samples because the environmental toxic compounds induce pH 
      changes in the acidic vesicles of biological structures that are used as 
      environmental biosensors.
FAU - Manente, Sabrina
AU  - Manente S
AD  - Department of Environmental Sciences, Ca' Foscari University of Venice, 
      Dorsoduro, Venice 30123, Italy.
FAU - De Pieri, Silvia
AU  - De Pieri S
FAU - Iero, Alessandra
AU  - Iero A
FAU - Rigo, Chiarafrancesca
AU  - Rigo C
FAU - Bragadin, Marcantonio
AU  - Bragadin M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20080917
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Coloring Agents)
RN  - 0 (Water Pollutants, Chemical)
RN  - 261QK3SSBH (Neutral Red)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - *Biosensing Techniques
MH  - Coloring Agents/*chemistry
MH  - Environmental Monitoring/*methods
MH  - Hydrogen-Ion Concentration
MH  - Lysosomes/chemistry
MH  - Neutral Red/*chemistry
MH  - Water Pollutants, Chemical/*analysis/toxicity
EDAT- 2008/10/07 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/10/07 09:00
PHST- 2008/08/01 00:00 [received]
PHST- 2008/09/04 00:00 [revised]
PHST- 2008/09/11 00:00 [accepted]
PHST- 2008/10/07 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/10/07 09:00 [entrez]
AID - S0003-2697(08)00626-X [pii]
AID - 10.1016/j.ab.2008.09.015 [doi]
PST - ppublish
SO  - Anal Biochem. 2008 Dec 15;383(2):316-9. doi: 10.1016/j.ab.2008.09.015. Epub 2008 
      Sep 17.