PMID- 18835249
OWN - NLM
STAT- MEDLINE
DCOM- 20081215
LR  - 20191210
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 377
IP  - 3
DP  - 2008 Dec 19
TI  - Differential subcellular localization of insulin receptor substrates depends on 
      C-terminal regions and importin beta.
PG  - 741-6
LID - 10.1016/j.bbrc.2008.09.106 [doi]
AB  - Insulin receptor substrates (IRSs) play essential roles in signal transduction of 
      insulin and insulin-like growth factors. Previously, we showed that IRS-3 is 
      localized to the nucleus as well as the cytosol, while IRS-1 and 2 are mainly 
      localized to the cytoplasm. In the present study, we found that importin beta 
      directly interacts with IRS-3 and is able to mediate nuclear transport of IRS-3. 
      Importin beta interacted with the pleckstrin homology domain, the phosphotyrosine 
      binding domain and the C-terminal region of IRS-3; indeed all of these fragments 
      exhibited predominant nuclear localization. By contrast, almost no interaction of 
      importin beta with IRS-1 and -2 was observed, and their C-terminal regions 
      displayed discrete spotty images in the cytosol. In addition, using chimeric 
      proteins between IRS-1 and IRS-3, we revealed that the C-terminal regions are the 
      main determinants of the differing subcellular localizations of IRS-1 and IRS-3.
FAU - Kabuta, Tomohiro
AU  - Kabuta T
AD  - Laboratory of Cell Regulation, Departments of Animal Sciences and Applied 
      Biological Chemistry, Graduate School of Agriculture and Life Sciences, The 
      University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Take, Kazumi
AU  - Take K
FAU - Kabuta, Chihana
AU  - Kabuta C
FAU - Hakuno, Fumihiko
AU  - Hakuno F
FAU - Takahashi, Shin-ichiro
AU  - Takahashi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081001
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (beta Karyopherins)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - EC 2.7.10.1 (Receptor, Insulin)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Cell Nucleus/metabolism
MH  - Chlorocebus aethiops
MH  - Cytosol/metabolism
MH  - Humans
MH  - Mutation
MH  - Phosphotyrosine/metabolism
MH  - Protein Structure, Tertiary
MH  - Rats
MH  - Receptor, Insulin/genetics/*metabolism
MH  - beta Karyopherins/genetics/*metabolism
EDAT- 2008/10/07 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/10/07 09:00
PHST- 2008/09/24 00:00 [received]
PHST- 2008/09/25 00:00 [accepted]
PHST- 2008/10/07 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/10/07 09:00 [entrez]
AID - S0006-291X(08)01890-1 [pii]
AID - 10.1016/j.bbrc.2008.09.106 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2008 Dec 19;377(3):741-6. doi: 
      10.1016/j.bbrc.2008.09.106. Epub 2008 Oct 1.