PMID- 1883525
OWN - NLM
STAT- MEDLINE
DCOM- 19911008
LR  - 20220227
IS  - 1015-2008 (Print)
IS  - 1015-2008 (Linking)
VI  - 59
IP  - 4
DP  - 1991
TI  - Regulation of M-CSF expression by M-CSF: role of protein kinase C and 
      transcription factor NF kappa B.
PG  - 284-8
AB  - Macrophage-colony-stimulating factor (M-CSF), also referred to as CSF-1, 
      regulates the survival, growth, differentiation and functional activity of 
      monocytes by binding to a single class of high-affinity cell surface receptors, 
      known to be the product of the c-fms protooncogene. The detection of both M-CSF 
      and c-fms expression by cells of the monocyte lineage has suggested that M-CSF 
      may act by an autocrine mechanism. Interestingly, it has been shown that M-CSF 
      can induce the expression of its own gene. Although sensitivity to M-CSF can be 
      modulated by regulation of receptor expression and function, M-CSF responsiveness 
      is largely determined at a postreceptor level. To date, little is known about the 
      intracellular pathway of M-CSF signal transduction. We have therefore 
      investigated the changes in protein kinase C (PKC) activity upon exposure of 
      monocytes to M-CSF. We show that M-CSF activates and translocates PKC. Inhibition 
      of PKC by the isoquinoline derivative H7 abolishes induction of M-CSF by M-CSF. 
      Furthermore, activation of PKC was pertussis-toxin-sensitive and was associated 
      with the detection of an NF kappa B protein in nuclear extracts of M-CSF-induced 
      blood monocytes but not in monocytes exposed to medium treatment only. The 
      results suggest that M-CSF induction of M-CSF involves G proteins, PKC and NF 
      kappa B.
FAU - Brach, M A
AU  - Brach MA
AD  - Department of Internal Medicine 1, University of Freiburg Medical Center, FRG.
FAU - Henschler, R
AU  - Henschler R
FAU - Mertelsmann, R H
AU  - Mertelsmann RH
FAU - Herrmann, F
AU  - Herrmann F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Pathobiology
JT  - Pathobiology : journal of immunopathology, molecular and cellular biology
JID - 9007504
RN  - 0 (DNA Probes)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Base Sequence
MH  - DNA/genetics
MH  - DNA Probes
MH  - Gene Expression Regulation
MH  - Humans
MH  - In Vitro Techniques
MH  - Macrophage Colony-Stimulating Factor/*biosynthesis/genetics/pharmacology
MH  - Molecular Sequence Data
MH  - Monocytes/drug effects/metabolism
MH  - NF-kappa B/*metabolism
MH  - Protein Kinase C/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Transcription, Genetic
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1159/000163664 [doi]
PST - ppublish
SO  - Pathobiology. 1991;59(4):284-8. doi: 10.1159/000163664.