PMID- 18837092
OWN - NLM
STAT- MEDLINE
DCOM- 20090402
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 14
IP  - 37
DP  - 2008 Oct 7
TI  - Characterization of hepatic progenitors from human fetal liver during second 
      trimester.
PG  - 5730-7
AB  - AIM: To enrich hepatic progenitors using epithelial cell adhesion molecule 
      (EpCAM) as a marker from human fetal liver and investigate the expression of 
      human leukocyte antigen (HLA) and their markers associated with hepatic 
      progenitor cells. METHODS: EpCAM +ve cells were isolated using magnetic cell 
      sorting (MACS) from human fetuses (n = 10) at 15-25 wk gestation. Expression of 
      markers for hepatic progenitors such as albumin, alpha-fetoprotein (AFP), CD29 
      (integrin beta1), CD49f (integrin alpha6) and CD90 (Thy 1) was studied by using 
      flow cytometry, immunocytochemistry and RT-PCR; HLA class I (A, B, C) and class 
      II (DR) expression was studied by flow cytometry only. RESULTS: FACS analysis 
      indicated that EpCAM +ve cells were positive for CD29, CD49f, CD90, CD34, HLA 
      class I, albumin and AFP but negative for HLA class II (DR) and CD45. RT PCR 
      showed that EpCAM +ve cells expressed liver epithelial markers (CK18), biliary 
      specific marker (CK19) and hepatic markers (albumin, AFP). On immunocytochemical 
      staining, EpCAM +ve cells were shown positive signals for CK18 and albumin. 
      CONCLUSION: Our study suggests that these EpCAM +ve cells can be used as hepatic 
      progenitors for cell transplantation with a minimum risk of alloreactivity and 
      these cells may serve as a potential source for enrichment of hepatic progenitor.
FAU - Rao, Mekala-Subba
AU  - Rao MS
AD  - Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
FAU - Khan, Aleem-Ahmed
AU  - Khan AA
FAU - Parveen, Nyamath
AU  - Parveen N
FAU - Habeeb, Mohammed-Aejaz
AU  - Habeeb MA
FAU - Habibullah, Chittoor-Mohammed
AU  - Habibullah CM
FAU - Pande, Gopal
AU  - Pande G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (AFP protein, human)
RN  - 0 (Albumins)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (EPCAM protein, human)
RN  - 0 (Epithelial Cell Adhesion Molecule)
RN  - 0 (HLA Antigens)
RN  - 0 (Integrin alpha6)
RN  - 0 (Integrin beta1)
RN  - 0 (Thy-1 Antigens)
RN  - 0 (alpha-Fetoproteins)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Albumins/metabolism
MH  - Antigens, Neoplasm/metabolism
MH  - Cell Adhesion Molecules/metabolism
MH  - Cells, Cultured
MH  - Epithelial Cell Adhesion Molecule
MH  - Female
MH  - Fetal Stem Cells/immunology/*metabolism
MH  - Flow Cytometry
MH  - HLA Antigens/metabolism
MH  - Hepatocytes/immunology/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunomagnetic Separation
MH  - Integrin alpha6/metabolism
MH  - Integrin beta1/metabolism
MH  - Keratins/metabolism
MH  - Liver/embryology/immunology/*metabolism
MH  - Phenotype
MH  - Pregnancy
MH  - Pregnancy Trimester, Second
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Thy-1 Antigens/metabolism
MH  - alpha-Fetoproteins/metabolism
PMC - PMC2748210
EDAT- 2008/10/07 09:00
MHDA- 2009/04/03 09:00
PMCR- 2008/10/07
CRDT- 2008/10/07 09:00
PHST- 2008/10/07 09:00 [pubmed]
PHST- 2009/04/03 09:00 [medline]
PHST- 2008/10/07 09:00 [entrez]
PHST- 2008/10/07 00:00 [pmc-release]
AID - 10.3748/wjg.14.5730 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2008 Oct 7;14(37):5730-7. doi: 10.3748/wjg.14.5730.