PMID- 18840994
OWN - NLM
STAT- MEDLINE
DCOM- 20090616
LR  - 20090407
IS  - 1422-6421 (Electronic)
IS  - 1422-6405 (Linking)
VI  - 189
IP  - 5
DP  - 2009
TI  - Novel system for degeneration of blood vessels by UV irradiation and subsequent 
      regeneration using chick bone marrow cells.
PG  - 348-55
LID - 10.1159/000162494 [doi]
AB  - Recently, many results have been reported regarding the pluripotency of bone 
      marrow cells (BMCs) with the aim of benefiting regenerative medicine for humans. 
      Particularly, vessel formation by hematopoietic stem cells or vascular 
      endothelial stem cells which were derived from bone marrow has received 
      considerable interest, since the mechanism of vessel formation has been found to 
      be involved in neoangiogenesis of serious diseases such as cancer. Most work on 
      neoangiogenesis and regeneration has involved mammalian experimental systems, 
      however the avian model is useful since the process of neoangiogenesis and 
      regeneration of vessels can be observed with the whole embryo culture system. We 
      have established a novel system using early chick embryos, where a portion of 
      blood vessels are degenerated by UV irradiation, and vessel regeneration is then 
      studied. Incubated embryos were partially covered with aluminum foil, from the 
      embryonic body to the dorsal marginal vein, and irradiated with UV for 1 min. 
      Donor BMCs were obtained from the femurs and tibias of chicks aged 10 days, 
      fluorescently labeled with PKH26 and injected into the anterior vitelline vein of 
      the recipients. In BMC-treated embryos the donor BMCs were observed around the 
      UV-degenerated vessels, and regeneration of blood vessels occurred, in contrast 
      to the untreated embryos. These results indicate that avian BMCs have the ability 
      to participate in vessel regeneration, and the avian model used here may be a 
      useful tool for studies of vessel neoangiogenesis and repair.
CI  - 2008 S. Karger AG, Basel.
FAU - Usui, Fumitake
AU  - Usui F
AD  - Faculty of Agriculture, Shinshu University, Nagano, Japan.
FAU - Yamamoto, Yasuhiro
AU  - Yamamoto Y
FAU - Nakamura, Yoshiaki
AU  - Nakamura Y
FAU - Ono, Tamao
AU  - Ono T
FAU - Kagami, Hiroshi
AU  - Kagami H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081005
PL  - Switzerland
TA  - Cells Tissues Organs
JT  - Cells, tissues, organs
JID - 100883360
SB  - IM
MH  - Animals
MH  - Blood Vessels/growth & development/*physiology/*radiation effects
MH  - Bone Marrow Cells/*cytology
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Chickens
MH  - Regeneration/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Engineering/methods
MH  - Ultraviolet Rays
EDAT- 2008/10/09 09:00
MHDA- 2009/06/17 09:00
CRDT- 2008/10/09 09:00
PHST- 2007/11/26 00:00 [accepted]
PHST- 2008/10/09 09:00 [pubmed]
PHST- 2009/06/17 09:00 [medline]
PHST- 2008/10/09 09:00 [entrez]
AID - 000162494 [pii]
AID - 10.1159/000162494 [doi]
PST - ppublish
SO  - Cells Tissues Organs. 2009;189(5):348-55. doi: 10.1159/000162494. Epub 2008 Oct 
      5.