PMID- 18841572
OWN - NLM
STAT- MEDLINE
DCOM- 20090325
LR  - 20081008
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 25
IP  - 5
DP  - 2008 Oct
TI  - [The mutation and expression of MUS81 gene in laryngeal squamous cell carcinoma].
PG  - 560-5
AB  - OBJECTIVE: To investigate the association of mutations and expression of MUS81 
      gene with the tumorigenesis and progression of laryngeal squamous cell carcinoma 
      (LSCC). METHODS: PCR-SSCP and DNA sequencing were carried out to examine 
      mutations at exons 9 and 10 of MUS81 gene in 42 LSCC samples, with paired 
      adjacent normal laryngeal tissues (PANLs) as control. Semi-quantitative RT-PCR 
      and Western blot were used to detect the expression of MUS81 gene in the 
      specimens. RESULTS: No mutation was detected in the control group. Among the 42 
      LSCC specimens, nineteen (45.2%) were found to harbor mutations, including 
      11(26.2%) occurring within exon 9, and 8 (19%) within exon 10. Seventeen (40.48%) 
      samples showed lower mRNA level of the MUS81 gene (P<0.01), and same proportion 
      of samples had lower protein level (P<0.01), suggesting that MUS81 gene was 
      similarly down-regulated at both mRNA and protein levels in the LSCC samples. 
      Furthermore, mutations of MUS81 gene did not significantly correlate with TNM 
      stages, age and lymphoid node metastasis (P>0.05). Nor did the expression of 
      MUS81 gene with the TNM stages, age and lymphoid node metastasis in LSCC 
      (P>0.05). CONCLUSION: Mutations and abnormal expression of MUS81 gene in the LSCC 
      tissues were observed, which suggested that abnormalities of MUS81 gene may play 
      an important role in the tumorigenesis of LSCC.
FAU - Li, Chanyuan
AU  - Li C
AD  - Department of Medical Genetics, China Medical University, Shenyang, Liaoning 
      110001, P.R. China.
FAU - Wang, Shuyun
AU  - Wang S
FAU - Yuan, Haiming
AU  - Yuan H
FAU - Li, Wei
AU  - Li W
FAU - Li, Zizheng
AU  - Li Z
FAU - Li, Xiaoyu
AU  - Li X
FAU - Guo, Xing
AU  - Guo X
FAU - Li, Fucai
AU  - Li F
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.- (Endonucleases)
RN  - EC 3.1.- (MUS81 protein, human)
SB  - IM
MH  - Age Factors
MH  - Base Sequence
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - DNA-Binding Proteins/*genetics
MH  - Endonucleases/*genetics
MH  - Exons/genetics
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Lymphatic Metastasis/genetics
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - Neoplasm Staging
MH  - RNA, Messenger/genetics/metabolism
EDAT- 2008/10/09 09:00
MHDA- 2009/03/26 09:00
CRDT- 2008/10/09 09:00
PHST- 2008/10/09 09:00 [pubmed]
PHST- 2009/03/26 09:00 [medline]
PHST- 2008/10/09 09:00 [entrez]
AID - 940625122 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008 Oct;25(5):560-5.