PMID- 18845333
OWN - NLM
STAT- MEDLINE
DCOM- 20090122
LR  - 20121115
IS  - 0141-1136 (Print)
IS  - 0141-1136 (Linking)
VI  - 66
IP  - 5
DP  - 2008 Dec
TI  - Semipermeable membrane devices link site-specific contaminants to effects: Part 1 
      - Induction of CYP1A in rainbow trout from contaminants in Prince William Sound, 
      Alaska.
PG  - 477-86
LID - 10.1016/j.marenvres.2008.07.001 [doi]
AB  - Extracts from semi-permeable membrane devices (SPMDs) deployed on beaches in 
      Prince William Sound (PWS), Alaska, were used to evaluate if complex contaminant 
      mixtures from different sources can be distinguished by the resulting cytochrome 
      P450 1A (CYP1A) activity in exposed test animals. Deployment sites included 
      canneries, salmon hatcheries, and beaches where lingering oil remains from 
      discharges during the 1964 earthquake or the 1989 Exxon Valdez oil spill. Other 
      sites were selected at random to evaluate region-wide contaminant inputs or were 
      located in salmon streams to evaluate contaminants carried and released by 
      migrating salmon carcasses following reproduction. Following standard deployments 
      of approximately 28 d, an aliquot of the accumulated contaminants was 
      intraperitoneally injected without cleanup into juvenile rainbow trout 
      (Oncorhynchus mykiss). After 2 d and 7 d, the activity of CYP1A was measured by 
      the ethoxyresorufin-o-deethylase (EROD) assay. Exposure to extracts from the 
      oiled sites and one hatchery site with numerous creosote pilings elicited strong 
      EROD responses, whereas fish exposed to salmon stream extracts elicited weak but 
      significant responses during late summer compared to late spring. Responses from 
      the other sites were not significant, indicating contaminants from these sources 
      are unlikely to cause CYP1A induction in resident biota. Rather than simply 
      assessing extant contaminants, this method evaluates the potency of the different 
      sites for bringing about aryl hydrocarbon receptor responses in resident biota.
FAU - Springman, Kathrine R
AU  - Springman KR
AD  - University of California Davis, Davis, CA, USA. krspringman@gmail.com
FAU - Short, Jeffrey W
AU  - Short JW
FAU - Lindeberg, Mandy R
AU  - Lindeberg MR
FAU - Maselko, Jacek M
AU  - Maselko JM
FAU - Khan, Colin
AU  - Khan C
FAU - Hodson, Peter V
AU  - Hodson PV
FAU - Rice, Stanley D
AU  - Rice SD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080708
PL  - England
TA  - Mar Environ Res
JT  - Marine environmental research
JID - 9882895
RN  - 0 (Water Pollutants, Chemical)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
CIN - Mar Environ Res. 2009 May-Jun;67(4-5):262-7; author reply 259-61. PMID: 19349074
MH  - Alaska
MH  - Animals
MH  - Aquaculture
MH  - Cytochrome P-450 CYP1A1/*metabolism
MH  - Environmental Monitoring/*instrumentation/*methods
MH  - Enzyme Induction/drug effects
MH  - Human Activities
MH  - Oceans and Seas
MH  - Oncorhynchus mykiss/*metabolism
MH  - Rivers
MH  - Water Pollutants, Chemical/*toxicity
EDAT- 2008/10/11 09:00
MHDA- 2009/01/23 09:00
CRDT- 2008/10/11 09:00
PHST- 2008/03/18 00:00 [received]
PHST- 2008/06/26 00:00 [revised]
PHST- 2008/07/01 00:00 [accepted]
PHST- 2008/10/11 09:00 [pubmed]
PHST- 2009/01/23 09:00 [medline]
PHST- 2008/10/11 09:00 [entrez]
AID - S0141-1136(08)00178-5 [pii]
AID - 10.1016/j.marenvres.2008.07.001 [doi]
PST - ppublish
SO  - Mar Environ Res. 2008 Dec;66(5):477-86. doi: 10.1016/j.marenvres.2008.07.001. 
      Epub 2008 Jul 8.