PMID- 18847511
OWN - NLM
STAT- MEDLINE
DCOM- 20090107
LR  - 20181113
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 9
DP  - 2008 Oct 13
TI  - Genomics and proteomics of immune modulatory effects of a butanol fraction of 
      echinacea purpurea in human dendritic cells.
PG  - 479
LID - 10.1186/1471-2164-9-479 [doi]
AB  - BACKGROUND: Echinacea spp. extracts and the derived phytocompounds have been 
      shown to induce specific immune cell activities and are popularly used as food 
      supplements or nutraceuticals for immuno-modulatory functions. Dendritic cells 
      (DCs), the most potent antigen presenting cells, play an important role in both 
      innate and adaptive immunities. In this study, we investigated the specific and 
      differential gene expression in human immature DCs (iDCs) in response to 
      treatment with a butanol fraction containing defined bioactive phytocompounds 
      extracted from stems and leaves of Echinacea purpurea, that we denoted 
      [BF/S+L/Ep]. RESULTS: Affymetrix DNA microarray results showed significant up 
      regulation of specific genes for cytokines (IL-8, IL-1beta, and IL-18) and 
      chemokines (CXCL 2, CCL 5, and CCL 2) within 4 h after [BF/S+L/Ep] treatment of 
      iDCs. Bioinformatics analysis of genes expressed in [BF/S+L/Ep]-treated DCs 
      revealed a key-signaling network involving a number of immune-modulatory 
      molecules leading to the activation of a downstream molecule, adenylate cyclase 
      8. Proteomic analysis showed increased expression of antioxidant and cytoskeletal 
      proteins after treatment with [BF/S+L/Ep] and cichoric acid. CONCLUSION: This 
      study provides information on candidate target molecules and molecular signaling 
      mechanisms for future systematic research into the immune-modulatory activities 
      of an important traditional medicinal herb and its derived phytocompounds.
FAU - Wang, Chien-Yu
AU  - Wang CY
AD  - Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan. 
      sally@mail.biowell.com.tw
FAU - Staniforth, Vanisree
AU  - Staniforth V
FAU - Chiao, Ming-Tsang
AU  - Chiao MT
FAU - Hou, Chia-Chung
AU  - Hou CC
FAU - Wu, Han-Ming
AU  - Wu HM
FAU - Yeh, Kuo-Chen
AU  - Yeh KC
FAU - Chen, Chun-Houh
AU  - Chen CH
FAU - Hwang, Pei-Ing
AU  - Hwang PI
FAU - Wen, Tuan-Nan
AU  - Wen TN
FAU - Shyur, Lie-Fen
AU  - Shyur LF
FAU - Yang, Ning-Sun
AU  - Yang NS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081013
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (Butanols)
RN  - 0 (Caffeic Acids)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Plant Extracts)
RN  - 0 (Succinates)
RN  - S4YY3V8YHD (chicoric acid)
SB  - IM
MH  - Butanols/chemistry
MH  - Caffeic Acids/pharmacology
MH  - Chemokines/genetics
MH  - Computational Biology
MH  - Cytokines/genetics
MH  - Cytoskeletal Proteins/metabolism
MH  - Dendritic Cells/*drug effects/immunology/*metabolism
MH  - Echinacea/*chemistry
MH  - Gene Expression/drug effects
MH  - Gene Expression Profiling
MH  - Genomics/*methods
MH  - Humans
MH  - Molecular Structure
MH  - Oligonucleotide Array Sequence Analysis
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Plant Leaves/chemistry
MH  - Plant Stems/chemistry
MH  - Proteomics/*methods
MH  - Succinates/pharmacology
PMC - PMC2571112
EDAT- 2008/10/14 09:00
MHDA- 2009/01/08 09:00
PMCR- 2008/10/13
CRDT- 2008/10/14 09:00
PHST- 2008/04/01 00:00 [received]
PHST- 2008/10/13 00:00 [accepted]
PHST- 2008/10/14 09:00 [pubmed]
PHST- 2009/01/08 09:00 [medline]
PHST- 2008/10/14 09:00 [entrez]
PHST- 2008/10/13 00:00 [pmc-release]
AID - 1471-2164-9-479 [pii]
AID - 10.1186/1471-2164-9-479 [doi]
PST - epublish
SO  - BMC Genomics. 2008 Oct 13;9:479. doi: 10.1186/1471-2164-9-479.