PMID- 18848629
OWN - NLM
STAT- MEDLINE
DCOM- 20090109
LR  - 20220110
IS  - 1095-9130 (Electronic)
IS  - 1046-2023 (Print)
IS  - 1046-2023 (Linking)
VI  - 46
IP  - 3
DP  - 2008 Nov
TI  - Measuring calcium dynamics in living cells with genetically encodable calcium 
      indicators.
PG  - 152-9
LID - 10.1016/j.ymeth.2008.09.015 [doi]
AB  - Genetically encoded calcium indicators (GECIs) allow researchers to measure 
      calcium dynamics in specific targeted locations within living cells. Such 
      indicators enable dissection of the spatial and temporal control of calcium 
      signaling processes. Here we review recent progress in the development of GECIs, 
      highlighting which indicators are most appropriate for measuring calcium in 
      specific organelles and localized domains in mammalian tissue culture cells. An 
      overview of recent approaches that have been undertaken to ensure that the GECIs 
      are minimally perturbed by the cellular environment is provided. Additionally, 
      the procedures for introducing GECIs into mammalian cells, conducting calcium 
      imaging experiments, and analyzing data are discussed. Because organelle-targeted 
      indicators often pose an additional challenge, we underscore strategies for 
      calibrating GECIs in these locations.
FAU - McCombs, Janet E
AU  - McCombs JE
AD  - Department of Chemistry and Biochemistry, University of Colorado, UCB 215, 76 
      Chemistry, Boulder, CO 80309-0215, USA.
FAU - Palmer, Amy E
AU  - Palmer AE
LA  - eng
GR  - T32 GM008759/GM/NIGMS NIH HHS/United States
GR  - T32 GM008759-08/GM/NIGMS NIH HHS/United States
GR  - T32 GM142607/GM/NIGMS NIH HHS/United States
GR  - T32 GM08759/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20081009
PL  - United States
TA  - Methods
JT  - Methods (San Diego, Calif.)
JID - 9426302
RN  - 0 (Indicators and Reagents)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Receptors, Calcium-Sensing)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*metabolism
MH  - *Calcium Signaling
MH  - Calibration
MH  - Cells, Cultured
MH  - Fluorescence Resonance Energy Transfer
MH  - Indicators and Reagents
MH  - Luminescent Proteins/metabolism
MH  - Models, Molecular
MH  - Organelles/metabolism
MH  - Receptors, Calcium-Sensing
MH  - Transfection/methods
PMC - PMC2654717
MID - NIHMS83586
EDAT- 2008/10/14 09:00
MHDA- 2009/01/10 09:00
PMCR- 2009/11/01
CRDT- 2008/10/14 09:00
PHST- 2008/03/06 00:00 [received]
PHST- 2008/09/12 00:00 [accepted]
PHST- 2008/10/14 09:00 [pubmed]
PHST- 2009/01/10 09:00 [medline]
PHST- 2008/10/14 09:00 [entrez]
PHST- 2009/11/01 00:00 [pmc-release]
AID - S1046-2023(08)00166-7 [pii]
AID - 10.1016/j.ymeth.2008.09.015 [doi]
PST - ppublish
SO  - Methods. 2008 Nov;46(3):152-9. doi: 10.1016/j.ymeth.2008.09.015. Epub 2008 Oct 9.