PMID- 18848653
OWN - NLM
STAT- MEDLINE
DCOM- 20090128
LR  - 20151119
IS  - 1873-2763 (Electronic)
IS  - 1873-2763 (Linking)
VI  - 44
IP  - 1
DP  - 2009 Jan
TI  - Differential mRNA expression profiles in proximal tibia of aged rats in response 
      to ovariectomy and low-Ca diet.
PG  - 46-52
LID - 10.1016/j.bone.2008.09.003 [doi]
AB  - Both ovariectomized animals and animals fed with Ca-depleted diets are commonly 
      used in vivo models for the investigation of osteoporosis-related bone loss. The 
      present study aimed to study the genomic responses of bone in aged female rats to 
      ovariectomy and dietary Ca deficiency in these models. Aged (11 months old) 
      Sprague-Dawley rats were subjected to bilateral ovariectomy or sham-operation and 
      fed with diets containing different dietary Ca content (LCD, 0.1% Ca or HCD, 1.2% 
      Ca) for 12 weeks. Serum and urine were collected for biochemical marker 
      measurement, and tibias were collected for bone mineral density (BMD) analysis by 
      pQCT as well as for gene expression analysis by real-time PCR. Ovariectomy 
      increased serum N-telopeptides of bone type I collagen (NTx) levels in aged rats 
      fed with HCD (P<0.05). In addition, ovariectomy reduced BMD and predicted bone 
      strength of tibial proximal metaphysis in aged rats fed with either LCD or HCD. 
      Dietary Ca deficiency did not alter serum bone-specific alkaline phosphatase 
      (BAP) or NTx levels, but induced a loss of BMD at tibia proximal metaphysis in 
      aged rats. Ovariectomy promoted the mRNA expression of alpha-1 type I collagen 
      (COL), osteoprotegerin (OPG) and receptor activator of nuclear factor-kappa B 
      ligand (RANKL); and inhibited the mRNA expression of cathepsin K and matrix 
      metalloproteinase-9 (MMP-9) in the proximal tibia of aged rats. Low-Ca diet 
      significantly up-regulated the mRNA expression of COL, core binding factor I 
      (Cbfa1), OPG and carbonic anhydrase II (CAII) in proximal tibia of aged rats. Our 
      study revealed that the genomic responses of bone in proximal tibia to 
      ovariectomy and dietary Ca deficiency were different. The bone loss induced by 
      ovariectomy appears to be mediated primarily by an increase in RANKL mRNA 
      expression; whereas the induction by dietary Ca restriction might be mediated by 
      the induction of carbonic anhydrase II expression.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, PR 
      China.
FAU - Dong, Xiao-Li
AU  - Dong XL
FAU - Leung, Ping-Chung
AU  - Leung PC
FAU - Wong, Man-Sau
AU  - Wong MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080920
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
RN  - 0 (Biomarkers)
RN  - 0 (Calcium, Dietary)
RN  - 0 (Osteoprotegerin)
RN  - 0 (RANK Ligand)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Aging/*genetics
MH  - Animals
MH  - Biomarkers/blood/urine
MH  - Calcium, Dietary/*pharmacology
MH  - *Diet
MH  - Female
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation/drug effects
MH  - Organ Specificity/drug effects
MH  - Osteoblasts/drug effects/metabolism
MH  - Osteoclasts/drug effects/metabolism
MH  - Osteoprotegerin/metabolism
MH  - *Ovariectomy
MH  - RANK Ligand/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tibia/*drug effects/*metabolism
MH  - Tomography, X-Ray Computed
MH  - Weight Gain/drug effects
EDAT- 2008/10/14 09:00
MHDA- 2009/01/29 09:00
CRDT- 2008/10/14 09:00
PHST- 2008/05/28 00:00 [received]
PHST- 2008/08/22 00:00 [revised]
PHST- 2008/09/09 00:00 [accepted]
PHST- 2008/10/14 09:00 [pubmed]
PHST- 2009/01/29 09:00 [medline]
PHST- 2008/10/14 09:00 [entrez]
AID - S8756-3282(08)00776-X [pii]
AID - 10.1016/j.bone.2008.09.003 [doi]
PST - ppublish
SO  - Bone. 2009 Jan;44(1):46-52. doi: 10.1016/j.bone.2008.09.003. Epub 2008 Sep 20.