PMID- 18849453
OWN - NLM
STAT- MEDLINE
DCOM- 20081229
LR  - 20190108
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 74
IP  - 23
DP  - 2008 Dec
TI  - Substrate-specific clades of active marine methylotrophs associated with a 
      phytoplankton bloom in a temperate coastal environment.
PG  - 7321-8
LID - 10.1128/AEM.01266-08 [doi]
AB  - Marine microorganisms that consume one-carbon (C(1)) compounds are poorly 
      described, despite their impact on global climate via an influence on aquatic and 
      atmospheric chemistry. This study investigated marine bacterial communities 
      involved in the metabolism of C(1) compounds. These communities were of relevance 
      to surface seawater and atmospheric chemistry in the context of a bloom that was 
      dominated by phytoplankton known to produce dimethylsulfoniopropionate. In 
      addition to using 16S rRNA gene fingerprinting and clone libraries to 
      characterize samples taken from a bloom transect in July 2006, seawater samples 
      from the phytoplankton bloom were incubated with (13)C-labeled methanol, 
      monomethylamine, dimethylamine, methyl bromide, and dimethyl sulfide to identify 
      microbial populations involved in the turnover of C(1) compounds, using DNA 
      stable isotope probing. The [(13)C]DNA samples from a single time point were 
      characterized and compared using denaturing gradient gel electrophoresis (DGGE), 
      fingerprint cluster analysis, and 16S rRNA gene clone library analysis. Bacterial 
      community DGGE fingerprints from (13)C-labeled DNA were distinct from those 
      obtained with the DNA of the nonlabeled community DNA and suggested some overlap 
      in substrate utilization between active methylotroph populations growing on 
      different C(1) substrates. Active methylotrophs were affiliated with Methylophaga 
      spp. and several clades of undescribed Gammaproteobacteria that utilized 
      methanol, methylamines (both monomethylamine and dimethylamine), and dimethyl 
      sulfide. rRNA gene sequences corresponding to populations assimilating 
      (13)C-labeled methyl bromide and other substrates were associated with members of 
      the Alphaproteobacteria (e.g., the family Rhodobacteraceae), the 
      Cytophaga-Flexibacter-Bacteroides group, and unknown taxa. This study expands the 
      known diversity of marine methylotrophs in surface seawater and provides a 
      comprehensive data set for focused cultivation and metagenomic analyses in the 
      future.
FAU - Neufeld, Josh D
AU  - Neufeld JD
AD  - Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, 
      United Kingdom.
FAU - Boden, Rich
AU  - Boden R
FAU - Moussard, Helene
AU  - Moussard H
FAU - Schafer, Hendrik
AU  - Schafer H
FAU - Murrell, J Colin
AU  - Murrell JC
LA  - eng
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PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081010
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Organic Chemicals)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Bacteria/*classification/isolation & purification/*metabolism
MH  - Carbon/metabolism
MH  - DNA Fingerprinting
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Genes, rRNA
MH  - Molecular Sequence Data
MH  - Organic Chemicals/*metabolism
MH  - Phylogeny
MH  - Phytoplankton/*microbiology
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/*microbiology
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
PMC - PMC2592898
EDAT- 2008/10/14 09:00
MHDA- 2008/12/30 09:00
PMCR- 2009/06/01
CRDT- 2008/10/14 09:00
PHST- 2008/10/14 09:00 [pubmed]
PHST- 2008/12/30 09:00 [medline]
PHST- 2008/10/14 09:00 [entrez]
PHST- 2009/06/01 00:00 [pmc-release]
AID - AEM.01266-08 [pii]
AID - 1266-08 [pii]
AID - 10.1128/AEM.01266-08 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2008 Dec;74(23):7321-8. doi: 10.1128/AEM.01266-08. Epub 
      2008 Oct 10.