PMID- 18852058
OWN - NLM
STAT- MEDLINE
DCOM- 20090109
LR  - 20151119
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 32
IP  - 12
DP  - 2008 Dec
TI  - Neuronal characteristics of amniotic fluid derived cells after adenoviral 
      transformation.
PG  - 1559-66
LID - 10.1016/j.cellbi.2008.09.002 [doi]
AB  - Efficient transformation of primary human amniocytes by E1 gene functions of 
      human adenovirus serotype 5 (Ad5) yield in stable cell lines, which exhibit 
      morphological features of epithelial like cells. A thorough investigation using 
      immunocytochemistry confirmed the expression of epithelial cell markers. The 
      analysis also revealed the expression of neuronal and glial marker proteins, such 
      as nestin, vimentin, A2B5 and GFAP. Using RT-PCR, transcripts of the neurotrophic 
      factors nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), 
      glial cell line derived neurotrophic factor (GDNF), and neurotrophin 3 (NT-3) 
      could be detected. Neurotrophic factors could also be detected in the cell 
      culture supernatants of transformed amniocytes. In line with previous 
      experimental data on a human Ad5 E1-transformed embryonal kidney cell line 
      (HEK-293), the results suggest a co-expression of epithelial and neuronal marker 
      proteins in E1-transformed human amniotic fluid derived cells and thus a 
      preferential transformation into neuronal-like cells.
FAU - Arnhold, S
AU  - Arnhold S
AD  - Department of Veterinary Anatomy, Histology and Embryology, Justus-Liebig, 
      University of Giessen, Frankfurter Street 98, 35392 Giessen, Germany. 
      stefan.arnhold@vetmed.uni-giessen.de
FAU - Post, C
AU  - Post C
FAU - Gluer, S
AU  - Gluer S
FAU - Hoopmann, M
AU  - Hoopmann M
FAU - Wenisch, S
AU  - Wenisch S
FAU - Volpers, C
AU  - Volpers C
FAU - Addicks, K
AU  - Addicks K
LA  - eng
PT  - Journal Article
DEP - 20080925
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Adenovirus E1 Proteins)
RN  - 0 (Biomarkers)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Adenovirus E1 Proteins/genetics
MH  - Amnion/*cytology/metabolism
MH  - Biomarkers/analysis/metabolism
MH  - Cell Differentiation/genetics
MH  - Cell Line, Transformed
MH  - Cells, Cultured
MH  - Epithelial Cells/*cytology/metabolism
MH  - Genetic Vectors/genetics
MH  - HeLa Cells
MH  - Humans
MH  - Nerve Growth Factors/genetics
MH  - Nerve Tissue Proteins/analysis/metabolism
MH  - Neurons/*cytology/metabolism
MH  - RNA, Messenger/analysis/metabolism
MH  - Stem Cells/cytology/metabolism
MH  - *Transformation, Genetic
EDAT- 2008/10/15 09:00
MHDA- 2009/01/10 09:00
CRDT- 2008/10/15 09:00
PHST- 2008/04/22 00:00 [received]
PHST- 2008/06/18 00:00 [revised]
PHST- 2008/09/10 00:00 [accepted]
PHST- 2008/10/15 09:00 [pubmed]
PHST- 2009/01/10 09:00 [medline]
PHST- 2008/10/15 09:00 [entrez]
AID - S1065-6995(08)00567-2 [pii]
AID - 10.1016/j.cellbi.2008.09.002 [doi]
PST - ppublish
SO  - Cell Biol Int. 2008 Dec;32(12):1559-66. doi: 10.1016/j.cellbi.2008.09.002. Epub 
      2008 Sep 25.