PMID- 18852264
OWN - NLM
STAT- MEDLINE
DCOM- 20090204
LR  - 20240109
IS  - 0021-9258 (Print)
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Linking)
VI  - 283
IP  - 49
DP  - 2008 Dec 5
TI  - Ascorbate peroxidase 1 plays a key role in the response of Arabidopsis thaliana 
      to stress combination.
PG  - 34197-203
LID - 10.1074/jbc.M806337200 [doi]
AB  - Within their natural habitat plants are subjected to a combination of different 
      abiotic stresses, each with the potential to exacerbate the damage caused by the 
      others. One of the most devastating stress combinations for crop productivity, 
      which frequently occurs in the field, is drought and heat stress. In this study 
      we conducted proteomic and metabolic analysis of Arabidopsis thaliana plants 
      subjected to a combination of drought and heat stress. We identified 45 different 
      proteins that specifically accumulated in Arabidopsis in response to the stress 
      combination. These included enzymes involved in reactive oxygen detoxification, 
      malate metabolism, and the Calvin cycle. The accumulation of malic enzyme during 
      the combined stress corresponded with enhanced malic enzyme activity, a decrease 
      in malic acid, and lower amounts of oxaloacetate, suggesting that malate 
      metabolism plays an important role in the response of Arabidopsis to the stress 
      combination. Cytosolic ascorbate peroxidase 1 (APX1) protein and mRNA accumulated 
      during the stress combination. When exposed to heat stress combined with drought, 
      an APX1-deficient mutant (apx1) accumulated more hydrogen peroxide and was 
      significantly more sensitive to the stress combination than wild type. In 
      contrast, mutants deficient in thylakoid or stromal/mitochondrial APXs were not 
      more sensitive to the stress combination than apx1 or wild type. Our findings 
      suggest that cytosolic APX1 plays a key role in the acclimation of plants to a 
      combination of drought and heat stress.
FAU - Koussevitzky, Shai
AU  - Koussevitzky S
AD  - Department of Biochemistry and Molecular Biology, University of Nevada, Reno, 
      Nevada 89557, USA.
FAU - Suzuki, Nobuhiro
AU  - Suzuki N
FAU - Huntington, Serena
AU  - Huntington S
FAU - Armijo, Leigh
AU  - Armijo L
FAU - Sha, Wei
AU  - Sha W
FAU - Cortes, Diego
AU  - Cortes D
FAU - Shulaev, Vladimir
AU  - Shulaev V
FAU - Mittler, Ron
AU  - Mittler R
LA  - eng
GR  - P20 RR16464-05/RR/NCRR NIH HHS/United States
GR  - RR-03-008/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20081013
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Chloroplast Proteins)
RN  - 0 (RNA, Messenger)
RN  - 059QF0KO0R (Water)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.11 (APXS protein, Arabidopsis)
RN  - EC 1.11.1.11 (Ascorbate Peroxidases)
SB  - IM
MH  - Arabidopsis/*enzymology
MH  - Arabidopsis Proteins
MH  - Ascorbate Peroxidases
MH  - Chloroplast Proteins
MH  - Chromatography, Liquid
MH  - Cytosol/enzymology/metabolism
MH  - Droughts
MH  - Gene Expression Regulation, Plant
MH  - Hot Temperature
MH  - Hydrogen Peroxide/chemistry
MH  - Mass Spectrometry
MH  - Models, Biological
MH  - Mutation
MH  - Peroxidases/*genetics/*physiology
MH  - Proteomics/methods
MH  - RNA, Messenger/metabolism
MH  - Time Factors
MH  - Water/chemistry
PMC - PMC2590703
EDAT- 2008/10/15 09:00
MHDA- 2009/02/05 09:00
PMCR- 2009/12/05
CRDT- 2008/10/15 09:00
PHST- 2008/10/15 09:00 [pubmed]
PHST- 2009/02/05 09:00 [medline]
PHST- 2008/10/15 09:00 [entrez]
PHST- 2009/12/05 00:00 [pmc-release]
AID - S0021-9258(20)65277-2 [pii]
AID - 34197 [pii]
AID - 10.1074/jbc.M806337200 [doi]
PST - ppublish
SO  - J Biol Chem. 2008 Dec 5;283(49):34197-203. doi: 10.1074/jbc.M806337200. Epub 2008 
      Oct 13.