PMID- 18852517
OWN - NLM
STAT- MEDLINE
DCOM- 20090203
LR  - 20131121
IS  - 1017-7825 (Print)
IS  - 1017-7825 (Linking)
VI  - 18
IP  - 9
DP  - 2008 Sep
TI  - Age- and area-dependent distinct effects of ethanol on Bax and Bcl-2 expression 
      in prenatal rat brain.
PG  - 1590-8
AB  - Cell proliferation and differentiation are critical processes in a developing 
      fetal rat brain, during which programmed cell death (PCD) also plays an important 
      role. One of the decisive factors for PCD is Bcl-2 family proteins, where Bax 
      induces cell death, whereas Bcl-2 acts as an inhibitor of PCD. As maternal 
      drinking is known to cause fetal alcohol syndrome (FAS) or malformation of the 
      fetal brain during pregnancy, the objective of the present study was to 
      investigate whether maternal ethanol exposure alters the PCD-related Bax and 
      Bcl-2 protein expression during fetal brain development. Pregnant female rats 
      were orally treated with 10% ethanol and the subsequent expressions of the Bax 
      and Bcl-2 proteins examined in the fetal brain, including the forebrain, 
      midbrain, and hindbrain, from gestational day (GD) 15.5 to GD 19.5, using Western 
      blots, in situ hybridization, and immunohistochemistry. With regard to the ratio 
      of Bcl-2 to Bax proteins (Bcl-2/Bax), the Bax protein was dominant in the 
      forebrain and midbrain of the control GD 15.5 fetuses, except for the hindbrain, 
      when compared with the respective ethanol-treated groups. Moreover, Bcl-2 became 
      dominant in the midbrain of the control GD 17.5 fetuses when compared with the 
      ethanoltreated group, representing an alternation of the natural PCD process by 
      ethanol. Furthermore, a differential expression of the Bcl-2 and Bax proteins was 
      found in the differentiating and migrating zones of the cortex, hippocampus, 
      thalamus, and cerebellum. Thus, when taken together, the present results suggest 
      that ethanol affects PCD in the cell differentiation and migration zones of the 
      prenatal rat brain by modulating Bax and Bcl-2 expression in an age- and 
      area-dependent manner. Therefore, this is the first evidence that ethanol may 
      alter FAS-associated embryonic brain development through the alteration of Bax 
      and Bcl-2 expression.
FAU - Lee, Hae Young
AU  - Lee HY
AD  - Division of Life Science and Applied Life Science (Brain Korea 21), Gyeongsang 
      National University, Jinju 660-701, Korea.
FAU - Naha, Nibedita
AU  - Naha N
FAU - Kim, Jong Hun
AU  - Kim JH
FAU - Jo, Mi Ja
AU  - Jo MJ
FAU - Min, Kwan Sik
AU  - Min KS
FAU - Seong, Hwan Hoo
AU  - Seong HH
FAU - Shin, Dong Hoon
AU  - Shin DH
FAU - Kim, Myeong Ok
AU  - Kim MO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (Bax protein, rat)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Apoptosis
MH  - Brain/drug effects/*embryology/*metabolism
MH  - Ethanol/administration & dosage/*toxicity
MH  - Female
MH  - Fetal Alcohol Spectrum Disorders/metabolism
MH  - Gene Expression Regulation, Developmental/*drug effects
MH  - Gestational Age
MH  - Immunohistochemistry
MH  - Pregnancy
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - bcl-2-Associated X Protein/genetics/*metabolism
EDAT- 2008/10/15 09:00
MHDA- 2009/02/04 09:00
CRDT- 2008/10/15 09:00
PHST- 2008/10/15 09:00 [pubmed]
PHST- 2009/02/04 09:00 [medline]
PHST- 2008/10/15 09:00 [entrez]
AID - 7523 [pii]
PST - ppublish
SO  - J Microbiol Biotechnol. 2008 Sep;18(9):1590-8.