PMID- 18852655
OWN - NLM
STAT- MEDLINE
DCOM- 20081117
LR  - 20090422
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 86
IP  - 7
DP  - 2008 Oct 15
TI  - Mimetic human leukocyte antigen epitopes: shown by monoclonal antibodies and 
      extra antibodies produced on transplantation.
PG  - 912-8
LID - 10.1097/TP.0b013e318183783b [doi]
AB  - BACKGROUND: Transplant patients often produce human leukocyte antigen (HLA) 
      antibodies against their donors and produce more specificities than can be 
      accounted for by HLA antigen mismatches. We theorize that the presence of extra, 
      otherwise unexplainable specificities could be accounted for if antibodies 
      reacted to more than one epitope (primary and mimetic) on distinct HLA molecules. 
      The theory states that mimetic epitopes consist of the same three amino acids 
      that comprise the primary, sterically placed approximately the same distance 
      apart as are the corresponding amino acids of the primary. METHODS: A mimetic 
      epitope table containing all primary epitopes and corresponding mimetic epitopes 
      was built. Then, the HLA specificities of monoclonal and single patient 
      antibodies were determined. These specificities that could not be defined by 
      unique position or amino acid epitopes alone were then used to query the mimetic 
      epitope table. RESULTS: A single antibody from a transplant patient and three 
      monoclonal antibodies produced reactions that can best be explained as the result 
      of one antibody reacting to the same amino acids at two distinct sites on the 
      molecule. Those position and amino acid combinations (pos/aa) are the primary and 
      mimetic epitopes. Using computerized methods of searching, mimetic epitopes were 
      found in five additional kidney transplant patients who produced 
      nondonor-specific antibodies in addition to donor-specific antibodies. 
      CONCLUSIONS: Epitopes on the HLA molecule that mimic the primary epitope have 
      been found. We suggest that these mimetic epitopes explain the additional 
      antibodies often found on HLA immunization resulting from allograft rejections, 
      pregnancies, and transfusions.
FAU - Sasaki, Nori
AU  - Sasaki N
AD  - One Lambda Incorporated, 21001 Kittridge Street, Canoga Park, CA, USA. 
      chibita91303@gmail.com
FAU - Idica, Adam
AU  - Idica A
FAU - Terasaki, Paul I
AU  - Terasaki PI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (Isoantibodies)
SB  - IM
CIN - Transplantation. 2009 Apr 27;87(8):1262-3; author reply 1263. PMID: 19384177
MH  - Adsorption
MH  - Antibodies, Monoclonal/*blood/isolation & purification
MH  - Antibody Specificity
MH  - Epitopes/blood/isolation & purification
MH  - HLA Antigens/*blood/isolation & purification
MH  - Humans
MH  - Isoantibodies/*blood/isolation & purification
MH  - Kidney Transplantation/*immunology
MH  - Leukocytes/*immunology
EDAT- 2008/10/15 09:00
MHDA- 2008/11/18 09:00
CRDT- 2008/10/15 09:00
PHST- 2008/10/15 09:00 [pubmed]
PHST- 2008/11/18 09:00 [medline]
PHST- 2008/10/15 09:00 [entrez]
AID - 00007890-200810150-00007 [pii]
AID - 10.1097/TP.0b013e318183783b [doi]
PST - ppublish
SO  - Transplantation. 2008 Oct 15;86(7):912-8. doi: 10.1097/TP.0b013e318183783b.