PMID- 18854840
OWN - NLM
STAT- MEDLINE
DCOM- 20090223
LR  - 20220317
IS  - 1559-7016 (Electronic)
IS  - 0271-678X (Print)
IS  - 0271-678X (Linking)
VI  - 29
IP  - 2
DP  - 2009 Feb
TI  - Glibenclamide reduces inflammation, vasogenic edema, and caspase-3 activation 
      after subarachnoid hemorrhage.
PG  - 317-30
LID - 10.1038/jcbfm.2008.120 [doi]
AB  - Subarachnoid hemorrhage (SAH) causes secondary brain injury due to vasospasm and 
      inflammation. Here, we studied a rat model of mild-to-moderate SAH intended to 
      minimize ischemia/hypoxia to examine the role of sulfonylurea receptor 1 (SUR1) 
      in the inflammatory response induced by SAH. mRNA for Abcc8, which encodes SUR1, 
      and SUR1 protein were abundantly upregulated in cortex adjacent to SAH, where 
      tumor-necrosis factor-alpha (TNFalpha) and nuclear factor (NF)kappaB signaling 
      were prominent. In vitro experiments confirmed that Abcc8 transcription is 
      stimulated by TNFalpha. To investigate the functional consequences of SUR1 
      expression after SAH, we studied the effect of the potent, selective SUR1 
      inhibitor, glibenclamide. We examined barrier permeability (immunoglobulin G, IgG 
      extravasation), and its correlate, the localization of the tight junction 
      protein, zona occludens 1 (ZO-1). SAH caused a large increase in barrier 
      permeability and disrupted the normal junctional localization of ZO-1, with 
      glibenclamide significantly reducing both effects. In addition, SAH caused large 
      increases in markers of inflammation, including TNFalpha and NFkappaB, and 
      markers of cell injury or cell death, including IgG endocytosis and caspase-3 
      activation, with glibenclamide significantly reducing these effects. We conclude 
      that block of SUR1 by glibenclamide may ameliorate several pathologic effects 
      associated with inflammation that lead to cortical dysfunction after SAH.
FAU - Simard, J Marc
AU  - Simard JM
AD  - Department of Neurosurgery, University of Maryland School of Medicine, Baltimore, 
      Maryland 21201-1595, USA. msimard@smail.umaryland.edu
FAU - Geng, Zhihua
AU  - Geng Z
FAU - Woo, S Kyoon
AU  - Woo SK
FAU - Ivanova, Svetlana
AU  - Ivanova S
FAU - Tosun, Cigdem
AU  - Tosun C
FAU - Melnichenko, Ludmila
AU  - Melnichenko L
FAU - Gerzanich, Volodymyr
AU  - Gerzanich V
LA  - eng
GR  - R01 NS061808/NS/NINDS NIH HHS/United States
GR  - NS048260/NS/NINDS NIH HHS/United States
GR  - R01 HL082517/HL/NHLBI NIH HHS/United States
GR  - NS061808/NS/NINDS NIH HHS/United States
GR  - HL082517/HL/NHLBI NIH HHS/United States
GR  - R01 NS048260/NS/NINDS NIH HHS/United States
GR  - R01 HL082517-02/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20081015
PL  - United States
TA  - J Cereb Blood Flow Metab
JT  - Journal of cerebral blood flow and metabolism : official journal of the 
      International Society of Cerebral Blood Flow and Metabolism
JID - 8112566
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Abcc8 protein, rat)
RN  - 0 (Potassium Channels, Inwardly Rectifying)
RN  - 0 (Receptors, Drug)
RN  - 0 (Sulfonylurea Receptors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.22.- (Caspase 3)
RN  - SX6K58TVWC (Glyburide)
SB  - IM
EIN - J Cereb Blood Flow Metab. 2009 Apr;29(4):872
MH  - ATP-Binding Cassette Transporters/genetics/metabolism
MH  - Animals
MH  - Blood-Brain Barrier/drug effects
MH  - Brain Edema/*drug therapy/*enzymology/pathology
MH  - Carotid Artery Injuries
MH  - Carotid Artery, Internal
MH  - Caspase 3/*metabolism
MH  - Enzyme Activation/drug effects
MH  - Glyburide/*therapeutic use
MH  - Hypoxia/pathology
MH  - Inflammation/*drug therapy/enzymology/pathology
MH  - Male
MH  - Potassium Channels, Inwardly Rectifying/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Drug/genetics/metabolism
MH  - Subarachnoid Hemorrhage/*drug therapy/*enzymology/pathology
MH  - Sulfonylurea Receptors
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Up-Regulation/genetics
PMC - PMC2740919
MID - NIHMS128907
EDAT- 2008/10/16 09:00
MHDA- 2009/02/24 09:00
PMCR- 2009/09/10
CRDT- 2008/10/16 09:00
PHST- 2008/10/16 09:00 [pubmed]
PHST- 2009/02/24 09:00 [medline]
PHST- 2008/10/16 09:00 [entrez]
PHST- 2009/09/10 00:00 [pmc-release]
AID - jcbfm2008120 [pii]
AID - 10.1038/jcbfm.2008.120 [doi]
PST - ppublish
SO  - J Cereb Blood Flow Metab. 2009 Feb;29(2):317-30. doi: 10.1038/jcbfm.2008.120. 
      Epub 2008 Oct 15.