PMID- 18855767
OWN - NLM
STAT- MEDLINE
DCOM- 20081114
LR  - 20081015
IS  - 0736-6205 (Print)
IS  - 0736-6205 (Linking)
VI  - 45
IP  - 4
DP  - 2008 Oct
TI  - FISH glossary: an overview of the fluorescence in situ hybridization technique.
PG  - 385-6, 388, 390 passim
LID - 10.2144/000112811 [doi]
AB  - The introduction of FISH (fluorescence in situ hybridization) marked the 
      beginning of a new era for the study of chromosome structure and function. As a 
      combined molecular and cytological approach, the major advantage of this visually 
      appealing technique resides in its unique ability to provide an intermediate 
      degree of resolution between DNA analysis and chromosomal investigations while 
      retaining information at the single-cell level. Used to support large-scale 
      mapping and sequencing efforts related to the human genome project, FISH accuracy 
      and versatility were subsequently capitalized on in biological and medical 
      research, providing a wealth of diverse applications and FISH-based diagnostic 
      assays. The diversification of the original FISH protocol into the impressive 
      number of procedures available these days has been promoted throughout the years 
      by a number of interconnected factors: the improvement in sensitivity, 
      specificity and resolution, together with the advances in the fields of 
      fluorescence microscopy and digital imaging, and the growing availability of 
      genomic and bioinformatic resources. By assembling in a glossary format many of 
      the "acronymed" FISH applications published so far, this review intends to 
      celebrate the ability of FISH to re-invent itself and thus remain at the 
      forefront of biomedical research.
FAU - Volpi, Emanuela V
AU  - Volpi EV
AD  - Molecular Cytogenetics Laboratory, Wellcome Trust Centre for Human Genetics, 
      University of Oxford, Oxford, UK. evolpi@well.ox.ac.uk
FAU - Bridger, Joanna M
AU  - Bridger JM
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
SB  - IM
MH  - Animals
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
RF  - 237
EDAT- 2008/10/16 09:00
MHDA- 2008/11/15 09:00
CRDT- 2008/10/16 09:00
PHST- 2008/10/16 09:00 [pubmed]
PHST- 2008/11/15 09:00 [medline]
PHST- 2008/10/16 09:00 [entrez]
AID - 000112811 [pii]
AID - 10.2144/000112811 [doi]
PST - ppublish
SO  - Biotechniques. 2008 Oct;45(4):385-6, 388, 390 passim. doi: 10.2144/000112811.