PMID- 1890808
OWN - NLM
STAT- MEDLINE
DCOM- 19911011
LR  - 20071114
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 65
IP  - 3
DP  - 1991 Sep
TI  - Effect of simian immunodeficiency virus infection on tumor necrosis factor-alpha 
      production by alveolar macrophages.
PG  - 280-6
AB  - We studied the release of tumor necrosis factor-alpha (TNF alpha), a vital 
      immunoregulatory cytokine, by alveolar macrophages (M phi s) infected with simian 
      immunodeficiency virus (SIV) in vitro or collected from SIV-infected macaques. 
      For in vitro studies, M phi s were harvested by bronchoalveolar lavage from 5 
      normal animals and infected in flasks with SIV (10(4)TCID50/2.5 x 10(6) M phi s). 
      After 7 to 10 days, cytopathic effect was prominent and 68 +/- 2% of M phi s were 
      immunoreactive for p27 core protein. Uninfected (control) and SIV-infected M phi 
      s were then cultured for 24 hours in 96-well plates (10(5) M phi s/well) while 
      challenged with lipopolysaccharide (LPS; 100 micrograms/ml). TNF alpha was 
      assayed in culture supernatants by an enzyme-linked immunosorbent assay 
      (detection limit, 50 pg/ml) and results were expressed as pg TNF alpha/ml/10(3) M 
      phi s (mean +/- SEM). TNF alpha was not detected in unstimulated wells. TNF alpha 
      release by control and SIV-infected M phi s was similar (6.6 +/- 0.7 and 7.9 +/- 
      1.1 pg/ml/10(3) M phi s, respectively). We also studied TNF alpha release by 
      alveolar M phi s from 8 animals infected with SIV (3 asymptomatic, 5 with 
      acquired immune deficiency syndrome virus (AIDS]. One animal with AIDS had p27+ M 
      phi s. Alveolar M phi s from asymptomatic animals released significantly more TNF 
      alpha (10.3 +/- 1.1 pg/ml/10(3) M phi s) than did animals with AIDS or uninfected 
      macaques (5.2 +/- 0.8 and 7.0 +/- 0.6 pg/ml/10(3) M phi s, respectively) (p less 
      than 0.01). However, M phi s from monkeys with AIDS failed to respond to LPS 
      after 7 to 10 days in culture. In summary, in vitro infection with SIV does not 
      cause constitutive TNF alpha release or alter the response of cultured M phi s to 
      LPS. When kept in culture, M phi s collected from asymptomatic, SIV-infected 
      animals retain their response to LPS, whereas M phi s from animals with AIDS lose 
      the capacity to produce TNF alpha. Furthermore, M phi s cytokine production is 
      exaggerated before overt clinical disease, but not as a direct result of 
      infection with SIV.
FAU - Horvath, C J
AU  - Horvath CJ
AD  - New England Regional Primate Research Center, Harvard Medical School, 
      Southborough, Massachusetts.
FAU - Desrosiers, R C
AU  - Desrosiers RC
FAU - Sehgal, P K
AU  - Sehgal PK
FAU - King, N W
AU  - King NW
FAU - Ringler, D J
AU  - Ringler DJ
LA  - eng
GR  - 5T32RR07000/RR/NCRR NIH HHS/United States
GR  - AI25644/AI/NIAID NIH HHS/United States
GR  - AI29855/AI/NIAID NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
CIN - Lab Invest. 1991 Sep;65(3):259-61. PMID: 1890805
MH  - Animals
MH  - Cell Count
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Macaca
MH  - Macrophages/*metabolism/pathology
MH  - Pulmonary Alveoli/*metabolism/pathology
MH  - Simian Acquired Immunodeficiency Syndrome/*metabolism
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1991 Sep;65(3):280-6.