PMID- 18922747
OWN - NLM
STAT- MEDLINE
DCOM- 20090407
LR  - 20151119
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 875
IP  - 2
DP  - 2008 Nov 15
TI  - Simultaneous analysis of THC and its metabolites in blood using liquid 
      chromatography-tandem mass spectrometry.
PG  - 465-70
LID - 10.1016/j.jchromb.2008.09.032 [doi]
AB  - Cannabis is considered to be the most widely abused illicit drug in Europe. 
      Consequently, sensitive and specific analytical methods are needed for forensic 
      purposes and for cannabinoid pharmacokinetic and pharmacodynamic studies. A 
      simple, rapid and highly sensitive and specific method for the extraction and 
      quantification of Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy- 
      Delta(9)-tetrahydrocannabinol (11-OH-THC) and 11-nor-9-carboxy- 
      Delta(9)-tetrahydrocannabinol (THC-COOH) in blood is presented. The method was 
      fully validated according to international guidelines and comprises simultaneous 
      liquid-liquid extraction (LLE) of the three analytes with hexane:ethyl acetate 
      (90:10, v/v) into a single eluant followed by separation and quantification using 
      liquid chromatography-tandem mass spectrometry (LC-MS/MS). Chromatographic 
      separation was achieved using a XBridge C(18) column eluted isocratically with 
      methanol:0.1% formic acid (80:20, v/v). Selectivity of the method was achieved by 
      a combination of retention time, and two precursor-product ion transitions. The 
      use of the LLE was demonstrated to be highly effective and led to significant 
      decreases in the interferences present in the matrix. Validation of the method 
      was performed using 250 microL of blood. The method was linear over the range 
      investigated (0.5-40 microg/L for THC, 1-40 microg/L for 11-OH-THC, and 2-160 
      microg/L for THC-COOH) with excellent intra-assay and inter-assay precision; 
      relative standard deviations (RSDs) were <12% for THC and 11-OH-THC and <8% for 
      THC-COOH for certified quality control samples. The lower limit of quantification 
      was fixed at the lowest calibrator in the linearity experiments. No instability 
      was observed after repeated freezing and thawing or in processed samples. The 
      method was subsequently applied to 63 authentic blood samples obtained from 
      toxicology cases. The validation and actual sample analysis results show that 
      this method is rugged, precise, accurate, and well suited for routine analysis.
FAU - Del Mar Ramirez Fernandez, Maria
AU  - Del Mar Ramirez Fernandez M
AD  - Federal Public Service Justice, National Institute of Criminalistics and 
      Criminology, Chaussee de Vilvorde 100, 1120 Brussels, Belgium. 
      mariadelmar.ramirezfernandez@just.fgov.be
FAU - De Boeck, Gert
AU  - De Boeck G
FAU - Wood, Michelle
AU  - Wood M
FAU - Lopez-Rivadulla, Manuel
AU  - Lopez-Rivadulla M
FAU - Samyn, Nele
AU  - Samyn N
LA  - eng
PT  - Journal Article
DEP - 20081002
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 26108-40-7 (11-hydroxy-delta(9)-tetrahydrocannabinol)
RN  - 4TPC9E4A32 (11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid)
RN  - 7J8897W37S (Dronabinol)
SB  - IM
MH  - Analysis of Variance
MH  - Chromatography, Liquid/*methods
MH  - Dronabinol/*analogs & derivatives/*blood/metabolism
MH  - Drug Stability
MH  - Humans
MH  - Linear Models
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2008/10/17 09:00
MHDA- 2009/04/08 09:00
CRDT- 2008/10/17 09:00
PHST- 2008/05/20 00:00 [received]
PHST- 2008/09/19 00:00 [revised]
PHST- 2008/09/28 00:00 [accepted]
PHST- 2008/10/17 09:00 [pubmed]
PHST- 2009/04/08 09:00 [medline]
PHST- 2008/10/17 09:00 [entrez]
AID - S1570-0232(08)00724-1 [pii]
AID - 10.1016/j.jchromb.2008.09.032 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 15;875(2):465-70. doi: 
      10.1016/j.jchromb.2008.09.032. Epub 2008 Oct 2.