PMID- 18922887
OWN - NLM
STAT- MEDLINE
DCOM- 20090312
LR  - 20181113
IS  - 1931-857X (Print)
IS  - 1522-1466 (Electronic)
IS  - 1522-1466 (Linking)
VI  - 295
IP  - 6
DP  - 2008 Dec
TI  - Tumor necrosis factor-alpha induces renal vasoconstriction as well as natriuresis 
      in mice.
PG  - F1836-44
LID - 10.1152/ajprenal.90297.2008 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been implicated in the pathogenesis 
      of hypertension and renal injury. However, the direct effects of TNF-alpha on 
      renal hemodynamic and excretory function are not yet clearly defined. We examined 
      the renal responses to infusion of TNF-alpha (0.33 ng.g(-1).min(-1)) in 
      anesthetized mice. Renal blood flow (RBF) and glomerular filtration rate (GFR) 
      were determined by PAH and inulin clearance. The urine was collected from a 
      cannula inserted into the bladder. Following the 60-min control clearance period, 
      TNF-alpha infusion was initiated and 15 min were given for stabilization followed 
      by another 60-min clearance period. TNF-alpha alone (n = 7) caused decreases in 
      RBF (7.9 +/- 0.3 to 6.4 +/- 0.3 ml.min(-1).g(-1)) and GFR (1.04 +/- 0.06 to 0.62 
      +/- 0.08 ml.min(-1).g(-1)) as well as increases in absolute (0.8 +/- 0.3 to 1.4 
      +/- 0.3 micromol.min(-1).g(-1)) and fractional excretion of sodium (0.5 +/- 0.2 
      to 1.5 +/- 0.4%) without affecting arterial pressure. TNF-alpha also increased 
      8-isoprostane excretion (8.10 +/- 1.09 to 11.13 +/- 1.34 pg.min(-1).g(-1)). 
      Pretreatment with TNF-alpha blocker etanercept (5 mg/kg sc; 24 and 3 h before 
      TNF-alpha infusion; n = 6) abolished these responses. However, TNF-alpha induced 
      an increase in RBF and caused attenuation of the GFR reduction in mice pretreated 
      with superoxide (O(2)(-)) scavenger tempol (2 microg.g(-1).min(-1); n = 6). 
      Pretreatment with nitric oxide (NO) synthase inhibitor nitro-l-arginine methyl 
      ester (0.1 microg.g(-1).min(-1); n = 6) resulted in further enhancement in 
      vasoconstriction while natriuresis remained unaffected in response to TNF-alpha. 
      These data suggest that TNF-alpha induces renal vasoconstriction and 
      hypofiltration via enhancing the activity of O(2)(-) and thus reducing the 
      activity of NO. The natriuretic response to TNF-alpha is related to its direct 
      effects on tubular sodium reabsorption.
FAU - Shahid, Mohd
AU  - Shahid M
AD  - Dept. of Physiology, SL-39, Tulane Univ. Health Sciences Center, 1430 Tulane 
      Ave., New Orleans, LA 70112, USA.
FAU - Francis, Joseph
AU  - Francis J
FAU - Majid, Dewan S A
AU  - Majid DS
LA  - eng
GR  - HL-66432/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20081015
PL  - United States
TA  - Am J Physiol Renal Physiol
JT  - American journal of physiology. Renal physiology
JID - 100901990
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 27415-26-5 (8-epi-prostaglandin F2alpha)
RN  - 9NEZ333N27 (Sodium)
RN  - B7IN85G1HY (Dinoprost)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Animals
MH  - Blood Flow Velocity/drug effects
MH  - Dinoprost/analogs & derivatives/urine
MH  - Glomerular Filtration Rate/drug effects
MH  - Hemodynamics/drug effects
MH  - Infusions, Intravenous
MH  - Kidney/drug effects/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Natriuresis/*drug effects
MH  - Potassium/urine
MH  - Renal Circulation/drug effects
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium/urine
MH  - Tumor Necrosis Factor-alpha/administration & dosage/genetics/*pharmacology
MH  - Vasoconstriction/*drug effects
PMC - PMC2604828
EDAT- 2008/10/17 09:00
MHDA- 2009/03/13 09:00
PMCR- 2008/10/15
CRDT- 2008/10/17 09:00
PHST- 2008/10/17 09:00 [pubmed]
PHST- 2009/03/13 09:00 [medline]
PHST- 2008/10/17 09:00 [entrez]
PHST- 2008/10/15 00:00 [pmc-release]
AID - 90297.2008 [pii]
AID - F-90297-2008 [pii]
AID - 10.1152/ajprenal.90297.2008 [doi]
PST - ppublish
SO  - Am J Physiol Renal Physiol. 2008 Dec;295(6):F1836-44. doi: 
      10.1152/ajprenal.90297.2008. Epub 2008 Oct 15.