PMID- 18926852
OWN - NLM
STAT- MEDLINE
DCOM- 20090317
LR  - 20191210
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 155
IP  - 1
DP  - 2009 Jan
TI  - Rapid detection of porcine reproductive and respiratory syndrome virus by reverse 
      transcription loop-mediated isothermal amplification assay.
PG  - 55-60
LID - 10.1016/j.jviromet.2008.09.012 [doi]
AB  - A rapid detection assay based on reverse transcription loop-mediated isothermal 
      amplification (RT-LAMP) has been developed for detecting porcine reproductive and 
      respiratory syndrome virus (PRRSV). The RT-LAMP assay utilized a set of six 
      primers to amplify the open reading frame 6 (ORF6) of the PRRSV. The amplified 
      products were analyzed by agarose gel electrophoresis or visualized by 
      colorimetric method. The results demonstrated that the RT-LAMP assay detected all 
      22 different PRRSV isolates, had no cross-reaction with four other swine viruses 
      (i.e., PCV2, SIV, CSFV, and PEDV), and obtained a 91.3% sensitivity in 23 
      positive clinical samples in reference to the permissive cells-based virus 
      isolation procedure. Therefore, the RT-LAMP assay provides a specific and 
      sensitive means for detecting PRRSV in a simple, fast, and cost-effective manner. 
      Furthermore, the RT-LAMP assay can be performed in less well-equipped 
      laboratories as well as fields.
FAU - Li, Qiang
AU  - Li Q
AD  - State Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - Zhou, Qing-Feng
AU  - Zhou QF
FAU - Xue, Chun-Yi
AU  - Xue CY
FAU - Ma, Jing-Yun
AU  - Ma JY
FAU - Zhu, Dao-Zhong
AU  - Zhu DZ
FAU - Cao, Yong-Chang
AU  - Cao YC
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20081108
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Time Factors
EDAT- 2008/10/18 09:00
MHDA- 2009/03/18 09:00
CRDT- 2008/10/18 09:00
PHST- 2008/04/01 00:00 [received]
PHST- 2008/09/04 00:00 [revised]
PHST- 2008/09/15 00:00 [accepted]
PHST- 2008/10/18 09:00 [pubmed]
PHST- 2009/03/18 09:00 [medline]
PHST- 2008/10/18 09:00 [entrez]
AID - S0166-0934(08)00333-9 [pii]
AID - 10.1016/j.jviromet.2008.09.012 [doi]
PST - ppublish
SO  - J Virol Methods. 2009 Jan;155(1):55-60. doi: 10.1016/j.jviromet.2008.09.012. Epub 
      2008 Nov 8.