PMID- 18928056
OWN - NLM
STAT- MEDLINE
DCOM- 20081105
LR  - 20081020
IS  - 1222-3891 (Print)
IS  - 1222-3891 (Linking)
VI  - 66
IP  - 1-2
DP  - 2007 Jan-Jun
TI  - Bacterial product cantastim derived from Pseudomonas aeruginosa induces migration 
      and maturation of dendritic cells.
PG  - 5-9
AB  - Dendritic cells (DCs) play a pivotal role in linking innate and adaptive 
      immunity. Migration to the lymph nodes and maturation of DCs are crucial steps in 
      the initiation of specific immune responses. The bacterial product CANTASTIM (CS) 
      is a purified extract of Pseudomonas aeruginosa that induces non-specific 
      protection against bacterial infection, enhances macrophage effector functions 
      and modulates cytokines production. In this study, we used a mouse skin explant 
      culture model and human monocyte-derived DCs to study the effect of CS on the 
      migration and maturation of DCs, respectively. We noticed a significant increase 
      in the number of DCs which migrated from the skin explants when CS was added to 
      the culture medium. Also, CS was able to induce the expression of 
      maturation-associated marker CD83 on human monocyte-derived DCs. DC-based tumor 
      vaccines represent a promising approach for cancer immunotherapy and the 
      migration rate and maturation state of DCs are important parameters for their 
      clinical effectiveness. CS may be an attractive candidate to be tested for the 
      production of DC-based vaccine.
FAU - Caras, Iuliana
AU  - Caras I
AD  - Infection and Immunity Laboratory, Cantacuzino National Institute of Research and 
      Development for Microbiology and Immunology, Bucharest, Romania. 
      immuno@cantacuzino.ro
FAU - Tucureanu, Catalin
AU  - Tucureanu C
FAU - Lerescu, Lucian
AU  - Lerescu L
FAU - Salageanu, Aurora
AU  - Salageanu A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Romania
TA  - Roum Arch Microbiol Immunol
JT  - Roumanian archives of microbiology and immunology
JID - 9204717
RN  - 0 (Culture Media)
RN  - 0 (Phospholipids)
RN  - 0 (cantastim)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Cell Movement/*drug effects
MH  - Cells, Cultured
MH  - Culture Media
MH  - Dendritic Cells/cytology/*drug effects/immunology
MH  - Humans
MH  - Mice
MH  - Monocytes/cytology/drug effects/immunology
MH  - Phospholipids/metabolism/*pharmacology
MH  - Pseudomonas aeruginosa/*metabolism
MH  - Skin/cytology
EDAT- 2008/10/22 09:00
MHDA- 2008/11/06 09:00
CRDT- 2008/10/22 09:00
PHST- 2008/10/22 09:00 [pubmed]
PHST- 2008/11/06 09:00 [medline]
PHST- 2008/10/22 09:00 [entrez]
PST - ppublish
SO  - Roum Arch Microbiol Immunol. 2007 Jan-Jun;66(1-2):5-9.