PMID- 18930187
OWN - NLM
STAT- MEDLINE
DCOM- 20091103
LR  - 20220317
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 92
IP  - 4
DP  - 2009 Oct
TI  - FISH on sperm: spot-counting to stop counting? Not yet.
PG  - 1474-1480
LID - S0015-0282(08)03560-7 [pii]
LID - 10.1016/j.fertnstert.2008.07.1779 [doi]
AB  - OBJECTIVE: To evaluate the reliability and applicability of the spot-counting 
      system (Cytovision Spot AX workstation) which offers an alternative to the 
      tedious manual analysis of sperm fluorescence in situ hybridization (FISH). 
      DESIGN: Manual and automatic analyses were performed and compared. SETTING: 
      Universitat Autonoma de Barcelona. PATIENT(S): Twenty-four men who asked for 
      information on infertility showing different seminal parameters. INTERVENTION(S): 
      A semen sample for each patient was collected and prepared for FISH. MAIN OUTCOME 
      MEASURE(S): A dual-color FISH using specific probes for chromosomes 13 and 21 and 
      a triple-color FISH with centromeric probes for chromosomes 18, X, and Y were 
      used (Vysis). Standard FISH analysis was carried out. Automatic analysis was 
      subsequently performed using a Spot AX system. RESULT(S): Overall, we performed 
      120 comparisons. In 116 out of 120 (96.67%), the percentage of anomalies reported 
      using manual counting fell within the incidence detected using the automatic 
      system. In the remaining comparisons, statistical differences were detected (4 
      out of 120; 3.33%). Time consumed by the automatic analysis was always higher 
      than the manual one, being influenced by the characteristics of the preparations. 
      CONCLUSION(S): The spot-counting system has potential, but before the service is 
      ready to be offered, we still need to overcome some limitations associated with 
      it.
FAU - Molina, Oscar
AU  - Molina O
AD  - Unitat de Biologia Cel.lular (Facultat de Biociencies), Universitat Autonoma de 
      Barcelona, Bellaterra (Cerdanyola del Valles), Spain.
FAU - Sarrate, Zaida
AU  - Sarrate Z
AD  - Unitat de Biologia Cel.lular (Facultat de Biociencies), Universitat Autonoma de 
      Barcelona, Bellaterra (Cerdanyola del Valles), Spain.
FAU - Vidal, Francesca
AU  - Vidal F
AD  - Unitat de Biologia Cel.lular (Facultat de Biociencies), Universitat Autonoma de 
      Barcelona, Bellaterra (Cerdanyola del Valles), Spain.
FAU - Blanco, Joan
AU  - Blanco J
AD  - Unitat de Biologia Cel.lular (Facultat de Biociencies), Universitat Autonoma de 
      Barcelona, Bellaterra (Cerdanyola del Valles), Spain. Electronic address: 
      joan.blanco@uab.cat.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081017
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, Pair 21
MH  - Diploidy
MH  - Humans
MH  - Image Processing, Computer-Assisted/methods
MH  - In Situ Hybridization, Fluorescence/*instrumentation/*methods
MH  - Male
MH  - Reproducibility of Results
MH  - Scintillation Counting/instrumentation/*methods
MH  - Semen Analysis/*methods
MH  - Sex Chromosomes
MH  - Spermatozoa/ultrastructure
MH  - Uniparental Disomy/diagnosis
EDAT- 2008/10/22 09:00
MHDA- 2009/11/05 06:00
CRDT- 2008/10/22 09:00
PHST- 2008/06/19 00:00 [received]
PHST- 2008/07/18 00:00 [revised]
PHST- 2008/07/31 00:00 [accepted]
PHST- 2008/10/22 09:00 [pubmed]
PHST- 2009/11/05 06:00 [medline]
PHST- 2008/10/22 09:00 [entrez]
AID - S0015-0282(08)03560-7 [pii]
AID - 10.1016/j.fertnstert.2008.07.1779 [doi]
PST - ppublish
SO  - Fertil Steril. 2009 Oct;92(4):1474-1480. doi: 10.1016/j.fertnstert.2008.07.1779. 
      Epub 2008 Oct 17.