PMID- 18930838
OWN - NLM
STAT- MEDLINE
DCOM- 20090123
LR  - 20081125
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 657
IP  - 1
DP  - 2008 Nov 17
TI  - Chromosome aberration frequencies and chromosome instability in mice after 
      long-term exposure to low-dose-rate gamma-irradiation.
PG  - 19-25
LID - 10.1016/j.mrgentox.2008.09.006 [doi]
AB  - Chronological changes of chromosome aberration rates related to accumulated doses 
      in chronically exposed humans and animals at a low-dose-rate have not been well 
      studied. C3H female specific pathogen-free mice (8 weeks of age) were chronically 
      irradiated. Chromosome aberration rate in mouse splenocytes after long-term 
      exposure to low-dose-rate (LDR) gamma-rays was serially determined by 
      conventional Giemsa method. Incidence of dicentrics and centric rings increased 
      almost linearly up to 8000 mGy following irradiation for about 400 days at a LDR 
      of 20 mGy/day. Clear dose-rate effects were observed in the chromosome aberration 
      frequencies between dose rates of 20 mGy/day and 200 Gy/day. Furthermore, the 
      frequencies of complex aberrations increased as accumulated doses increased in 
      LDR irradiation. This trend was also observed for the incidences of micronuclei 
      and trisomies of chromosomes 5, 13 and 18 in splenocytes, detected by 
      micronucleus assay and metaphase fluorescence in situ hybridization (FISH) 
      method, respectively. Incidences of 2-4 micronuclei and trisomy increased in 
      mouse splenocytes after irradiation of 8000 mGy at a LDR of 20 mGy/day. These 
      complex chromosome aberrations and numerical chromosome aberrations seem to be 
      induced indirectly after radiation exposure and thus the results indicate that 
      continuous gamma-ray irradiation for 400 days at LDR of 20 mGy/day induced 
      chromosomal instability in mice. These results are important to evaluate the 
      biological effects of long-term exposure to LDR radiation in humans.
FAU - Tanaka, Kimio
AU  - Tanaka K
AD  - Department of Radiobiology, Institute for Environmental Sciences, Hacchazwa 
      2-121, Takahoko, Rokkasho, Kamikita, Aomori 039-3213, Japan. kmtanaka@ies.or.jp
FAU - Kohda, Atsushi
AU  - Kohda A
FAU - Toyokawa, Takuo
AU  - Toyokawa T
FAU - Ichinohe, Kazuaki
AU  - Ichinohe K
FAU - Oghiso, Yoichi
AU  - Oghiso Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080927
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
SB  - IM
MH  - Aneuploidy
MH  - Animals
MH  - *Chromosomal Instability
MH  - *Chromosome Aberrations
MH  - Gamma Rays/*adverse effects
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Micronucleus Tests
MH  - Radiation Dosage
MH  - Ring Chromosomes
MH  - Spleen/ultrastructure
EDAT- 2008/10/22 09:00
MHDA- 2009/01/24 09:00
CRDT- 2008/10/22 09:00
PHST- 2008/09/11 00:00 [received]
PHST- 2008/09/15 00:00 [accepted]
PHST- 2008/10/22 09:00 [pubmed]
PHST- 2009/01/24 09:00 [medline]
PHST- 2008/10/22 09:00 [entrez]
AID - S1383-5718(08)00266-0 [pii]
AID - 10.1016/j.mrgentox.2008.09.006 [doi]
PST - ppublish
SO  - Mutat Res. 2008 Nov 17;657(1):19-25. doi: 10.1016/j.mrgentox.2008.09.006. Epub 
      2008 Sep 27.