PMID- 18931381
OWN - NLM
STAT- MEDLINE
DCOM- 20091002
LR  - 20151119
IS  - 1475-3162 (Electronic)
IS  - 0003-4878 (Linking)
VI  - 52
IP  - 8
DP  - 2008 Nov
TI  - Physical and biochemical properties of airborne flour particles involved in 
      occupational asthma.
PG  - 727-37
LID - 10.1093/annhyg/men061 [doi]
AB  - Aerosol particles which deeply penetrate the human airways and which trigger 
      baker's asthma manifestations are known to represent only a part of flour and of 
      airborne particles found in bakeries. They were a major focus of this study. To 
      this end, aerosols were produced from different wheat and rye flours, using an 
      automatic generator designed for bronchial challenge. Particles were 
      characterized for their size distribution, their ability to be deposited in the 
      airways, their protein content, their histological composition and their 
      reactivity with immunoglobulin E (IgE) present in sera from asthmatic bakers. 
      Like dust particles collected in the bakery, the aerosols produced showed 
      increased protein content but decreased IgE reactive protein content when 
      compared to the corresponding bulk flours. The sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis analysis of these particles showed a 
      predominance of endosperm gluten proteins. Under scanning electron microscopy, 
      flour particles displayed various tissue fragments with entrapped large A-starch 
      and small B- or C-starch granules, whereas aerosol particles appeared primarily 
      as a mixture of the endosperm intracellular interstitial protein matrix and small 
      B- or C-starch granules free or still associated. These observations showed that 
      aerosols supposed to penetrate deeply the airways, mainly correspond to 
      intracellular fragments of endosperm cells enriched in gluten proteins but with 
      lower amount of allergens belonging to albumins or globulins.
FAU - Lauriere, Michel
AU  - Lauriere M
AD  - UMR206 de chimie biologique, AgroParisTech, INRA, 78850 Thiverval-Grignon, 
      France. michel.lauriere@grignon.inra.fr
FAU - Gorner, Peter
AU  - Gorner P
FAU - Bouchez-Mahiout, Isabelle
AU  - Bouchez-Mahiout I
FAU - Wrobel, Richard
AU  - Wrobel R
FAU - Breton, Christine
AU  - Breton C
FAU - Fabries, Jean-Francois
AU  - Fabries JF
FAU - Choudat, Dominique
AU  - Choudat D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081017
PL  - England
TA  - Ann Occup Hyg
JT  - The Annals of occupational hygiene
JID - 0203526
RN  - 0 (Aerosols)
RN  - 0 (Air Pollutants, Occupational)
SB  - IM
MH  - Adult
MH  - Aerosols
MH  - Air Pollutants, Occupational/*analysis
MH  - Asthma/*etiology
MH  - Environmental Monitoring/methods
MH  - Flour/adverse effects/*analysis
MH  - *Food Industry
MH  - Humans
MH  - Male
MH  - Microscopy, Electron, Scanning
MH  - Occupational Diseases/*etiology
MH  - Occupational Medicine/methods
MH  - Particle Size
MH  - Secale
MH  - Triticum
EDAT- 2008/10/22 09:00
MHDA- 2009/10/03 06:00
CRDT- 2008/10/22 09:00
PHST- 2008/10/22 09:00 [pubmed]
PHST- 2009/10/03 06:00 [medline]
PHST- 2008/10/22 09:00 [entrez]
AID - men061 [pii]
AID - 10.1093/annhyg/men061 [doi]
PST - ppublish
SO  - Ann Occup Hyg. 2008 Nov;52(8):727-37. doi: 10.1093/annhyg/men061. Epub 2008 Oct 
      17.