PMID- 18931486
OWN - NLM
STAT- MEDLINE
DCOM- 20081112
LR  - 20081020
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 122
IP  - 1
DP  - 2008
TI  - Construction, characterization and FISH mapping of a bacterial artificial 
      chromosome library of Chinese pangolin (Manis pentadactyla).
PG  - 55-60
LID - 10.1159/000151316 [doi]
AB  - Chinese pangolins as a representative species in the order Pholidota have highly 
      specified morphological characters and occupy an important place in the mammalian 
      phylogenetic tree. To obtain genomic data for this species, we have constructed a 
      bacterial artificial chromosome (BAC) library of Chinese pangolin. The library 
      contains 208,272 clones with an average insert size of 122.1 kb and represents 
      approximately eight times the Chinese pangolin haploid genome (if we assume that 
      the Chinese pangolins have a genome size similar to human). One hundred and 
      twenty randomly-selected BAC clones were mapped onto Chinese pangolin chromosomes 
      by fluorescence in situ hybridization (FISH), showing a largely unbiased 
      chromosomal distribution. Several clones containing repetitive DNA and ribosomal 
      DNA genes were also found. The BAC library and FISH mapped BAC clones are useful 
      resources for comparative genomics and cytogenetics of mammals and in particular, 
      the ongoing genome sequencing project of Chinese pangolins.
CI  - Copyright 2008 S. Karger AG, Basel.
FAU - Che, J
AU  - Che J
AD  - State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of 
      Zoology, Chinese Academy of Sciences, Kunming, P.R. China.
FAU - Wang, J
AU  - Wang J
FAU - Su, W
AU  - Su W
FAU - Ye, J
AU  - Ye J
FAU - Wang, Y
AU  - Wang Y
FAU - Nie, W
AU  - Nie W
FAU - Yang, F
AU  - Yang F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081014
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Ribosomal)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - China
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - DNA/genetics
MH  - DNA, Ribosomal/genetics
MH  - Gene Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mammals/*classification/*genetics
MH  - Phylogeny
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 2008/10/22 09:00
MHDA- 2008/11/13 09:00
CRDT- 2008/10/22 09:00
PHST- 2008/05/27 00:00 [accepted]
PHST- 2008/10/22 09:00 [pubmed]
PHST- 2008/11/13 09:00 [medline]
PHST- 2008/10/22 09:00 [entrez]
AID - 000151316 [pii]
AID - 10.1159/000151316 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2008;122(1):55-60. doi: 10.1159/000151316. Epub 2008 Oct 
      14.