PMID- 18931490
OWN - NLM
STAT- MEDLINE
DCOM- 20081112
LR  - 20081020
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 122
IP  - 1
DP  - 2008
TI  - Distribution of the tandem repeat sequences and karyotyping in cucumber (Cucumis 
      sativus L.) by fluorescence in situ hybridization.
PG  - 80-8
LID - 10.1159/000151320 [doi]
AB  - We analyzed repeat sequences composition in the genome of cucumber inbred line 
      9930 using whole-genome shotgun reads. The analysis showed that satellite DNA 
      sequences are the most dominant components in the cucumber genome. The 
      distribution pattern of several tandem repeat sequences (Type I/II, Type III and 
      Type IV) on cucumber chromosomes was visualized using fluorescence in situ 
      hybridization (FISH). The FISH signals of the Type III and 45S rDNA provide 
      useful cytogenetic markers, whose position and fluorescence intensity allow for 
      easy identification of all somatic metaphase chromosomes. A karyotype showing the 
      position and fluorescence intensity of several tandem repeat sequences is 
      constructed. The establishment of this FISH-based karyotype has created the basis 
      for the integration of molecular, genetic and cytogenetic maps in Cucumis sativus 
      and for the ultimate genome sequencing project as well.
CI  - Copyright 2008 S. Karger AG, Basel.
FAU - Han, Y H
AU  - Han YH
AD  - National Maize Improvement Center of China, Key Laboratory of Crop Genetic 
      Improvement and Genome Ministry of Agriculture, China Agricultural University, 
      Beijing, China.
FAU - Zhang, Z H
AU  - Zhang ZH
FAU - Liu, J H
AU  - Liu JH
FAU - Lu, J Y
AU  - Lu JY
FAU - Huang, S W
AU  - Huang SW
FAU - Jin, W W
AU  - Jin WW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081014
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA, Satellite)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Centromere/genetics
MH  - Chromosomes, Plant/genetics
MH  - Cucumis sativus/*genetics
MH  - DNA, Plant/*genetics
MH  - DNA, Ribosomal/genetics
MH  - DNA, Satellite/genetics
MH  - Genome, Plant
MH  - Heterochromatin/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Tandem Repeat Sequences
MH  - Telomere/genetics
EDAT- 2008/10/22 09:00
MHDA- 2008/11/13 09:00
CRDT- 2008/10/22 09:00
PHST- 2008/06/12 00:00 [accepted]
PHST- 2008/10/22 09:00 [pubmed]
PHST- 2008/11/13 09:00 [medline]
PHST- 2008/10/22 09:00 [entrez]
AID - 000151320 [pii]
AID - 10.1159/000151320 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2008;122(1):80-8. doi: 10.1159/000151320. Epub 2008 Oct 14.