PMID- 18931534
OWN - NLM
STAT- MEDLINE
DCOM- 20090109
LR  - 20081020
IS  - 1423-0291 (Electronic)
IS  - 1015-2008 (Linking)
VI  - 75
IP  - 5
DP  - 2008
TI  - Losses of chromosome arms 4q, 8p, 13q and gain of 8q are correlated with 
      increasing chromosomal instability in hepatocellular carcinoma.
PG  - 312-22
LID - 10.1159/000151712 [doi]
AB  - OBJECTIVE: Chromosomal instability is a key feature in hepatocellular carcinoma 
      (HCC). Array comparative genomic hybridization (aCGH) revealed recurring 
      structural aberrations, whereas fluorescence in situ hybridization (FISH) 
      indicated an increasing number of numerical aberrations in dedifferentiating HCC. 
      Therefore, we examined whether there was a correlation between structural and 
      numerical aberrations of chromosomal instability in HCC. METHODS AND RESULTS: 27 
      HCC (5 well, 10 moderately, 12 lower differentiated) already cytogenetically 
      characterized by aCGH were analyzed. FISH analysis using probes for chromosomes 
      1, 3, 7, 8 and 17 revealed 1.46-4.24 signals/nucleus, which correlated with the 
      histological grade (well vs. moderately,p < 0.0003; moderately vs. lower, p < 
      0.004). The number of chromosomes to each other was stable with exceptions only 
      seen for chromosome 8. Loss of 4q and 13q, respectively, were correlated with the 
      number of aberrations detected by aCGH (p < 0.001, p < 0.005; Mann-Whitney test). 
      Loss of 4q and gain of 8q were correlated with an increasing number of numerical 
      aberrations detected by FISH (p < 0.020, p < 0.031). Loss of 8p was correlated 
      with the number of structural imbalances seen in aCGH (p < 0.048), but not with 
      the number of numerical changes seen in FISH. CONCLUSION: We found that losses of 
      4q, 8p and 13q were closely correlated with an increasing number of aberrations 
      detected by aCGH, whereas a loss of 4q and a gain of 8q were also observed in the 
      context of polyploidization, the cytogenetic correlate of morphological 
      dedifferentiation.
CI  - Copyright 2008 S. Karger AG, Basel.
FAU - Hertz, Sabine
AU  - Hertz S
AD  - Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, 
      Germany.
FAU - Rothamel, Thomas
AU  - Rothamel T
FAU - Skawran, Britta
AU  - Skawran B
FAU - Giere, Christian
AU  - Giere C
FAU - Steinemann, Doris
AU  - Steinemann D
FAU - Flemming, Peer
AU  - Flemming P
FAU - Becker, Thomas
AU  - Becker T
FAU - Flik, Jacobus
AU  - Flik J
FAU - Wiese, Birgit
AU  - Wiese B
FAU - Soudah, Bisharah
AU  - Soudah B
FAU - Kreipe, Hans
AU  - Kreipe H
FAU - Schlegelberger, Brigitte
AU  - Schlegelberger B
FAU - Wilkens, Ludwig
AU  - Wilkens L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081015
PL  - Switzerland
TA  - Pathobiology
JT  - Pathobiology : journal of immunopathology, molecular and cellular biology
JID - 9007504
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Hepatocellular/*genetics/pathology
MH  - Child
MH  - *Chromosomal Instability
MH  - Chromosomes, Human, Pair 13/*genetics
MH  - Chromosomes, Human, Pair 4/*genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Liver Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Polyploidy
MH  - Young Adult
EDAT- 2008/10/22 09:00
MHDA- 2009/01/10 09:00
CRDT- 2008/10/22 09:00
PHST- 2008/02/21 00:00 [received]
PHST- 2008/06/06 00:00 [accepted]
PHST- 2008/10/22 09:00 [pubmed]
PHST- 2009/01/10 09:00 [medline]
PHST- 2008/10/22 09:00 [entrez]
AID - 000151712 [pii]
AID - 10.1159/000151712 [doi]
PST - ppublish
SO  - Pathobiology. 2008;75(5):312-22. doi: 10.1159/000151712. Epub 2008 Oct 15.