PMID- 18932269
OWN - NLM
STAT- MEDLINE
DCOM- 20090625
LR  - 20081104
IS  - 0006-3525 (Print)
IS  - 0006-3525 (Linking)
VI  - 91
IP  - 1
DP  - 2009 Jan
TI  - Spectroscopic detection and identification of infected cells with herpes viruses.
PG  - 61-7
LID - 10.1002/bip.21082 [doi]
AB  - Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and 
      Fourier transform infrared (FTIR) microspectroscopy were previously applied for 
      the identification of various biological samples. In the present study, normal 
      cells in culture and cells infected with herpes simplex virus type 2 (HSV-2) or 
      varicella-zoster virus (VZV) were analyzed by MALDI-TOF and FTIR microscopy. 
      Specific spectral biomarkers for rapid and reliable monitoring and identification 
      of infected cells and probably for the discrimination between these viruses were 
      searched. The results show consistent spectral peaks in all examined normal 
      uninfected human fibroblast cells both in MALDI-T0F and FTIR microscopy. In 
      HSV-2- or VZV-infected cells, two unique peaks for each appeared at m/z 5397 and 
      5813 or at m/z 3501 and 4951, respectively, in MALDI-TOF spectra. In addition, 
      several peaks that appeared in control uninfected cells at the region m/z 
      13,000-20,000 disappeared completely in all examined infected samples. When these 
      infected cells were examined by FTIR microscopy, a band at 859 cm(-1) in control 
      uninfected cells was significantly shifted to 854 cm(-1) in both HSV2- and 
      VZV-infected cells. In addition, phosphate levels were considerably increased in 
      all infected cells compared to normal uninfected cells. These parameters could be 
      used as a basis for developing a spectral method for the detection and 
      identification of cells infected with herpes viruses.
FAU - Erukhimovitch, Vitaly
AU  - Erukhimovitch V
AD  - Analytical Equipment Unit, Ben-Gurion University of the Negev, Beer-Sheva, 
      Israel.
FAU - Karpasasa, Mark
AU  - Karpasasa M
FAU - Huleihel, Mahmoud
AU  - Huleihel M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biopolymers
JT  - Biopolymers
JID - 0372525
RN  - 0 (Nucleic Acids)
RN  - 0 (Phosphates)
SB  - IM
MH  - Carbohydrate Metabolism
MH  - Cell Line
MH  - Fibroblasts/*virology
MH  - Herpesviridae/chemistry/*isolation & purification/*physiology
MH  - Humans
MH  - Kinetics
MH  - Nucleic Acids/metabolism
MH  - Phosphates/metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2008/10/22 09:00
MHDA- 2009/06/26 09:00
CRDT- 2008/10/22 09:00
PHST- 2008/10/22 09:00 [pubmed]
PHST- 2009/06/26 09:00 [medline]
PHST- 2008/10/22 09:00 [entrez]
AID - 10.1002/bip.21082 [doi]
PST - ppublish
SO  - Biopolymers. 2009 Jan;91(1):61-7. doi: 10.1002/bip.21082.