PMID- 18939892
OWN - NLM
STAT- MEDLINE
DCOM- 20090610
LR  - 20161018
IS  - 0730-7268 (Print)
IS  - 0730-7268 (Linking)
VI  - 28
IP  - 3
DP  - 2009 Mar
TI  - Disposition of perfluorinated acid isomers in Sprague-Dawley rats; part 1: single 
      dose.
PG  - 542-54
LID - 10.1897/08-239.1 [doi]
AB  - Perfluorinated acids (PFAs) and their precursors (PFA-precursors) exist in the 
      environment as linear and multiple branched isomers. These isomers are 
      hypothesized to have different biological properties, but no isomer-specific data 
      are currently available. The present study is the first in a two-part project 
      examining PFA isomer-specific uptake, tissue distribution, and elimination in a 
      rodent model. Seven male Sprague-Dawley rats were administered a single gavage 
      dose of approximately 500 microg/kg body weight perfluorooctane sulfonate 
      (C(8)F(17)SO(3)(-), PFOS), perfluorooctanoic acid (C(7)F(15)CO(2)H, PFOA), and 
      perfluorononanoic acid (C(8)F(17)CO(2)H, PFNA) and 30 microg/kg body weight 
      perfluorohexane sulfonate (C(6)F(13)SO(3)(-), PFHxS). Over the subsequent 38 d, 
      urine, feces, and tail-vein blood samples were collected intermittently, while 
      larger blood volumes and tissues were collected on days 3 and 38 for isomer 
      analysis by high-performance liquid chromatography-tandem mass spectrometry 
      (HPLC-MS/MS). For all PFAs, branched isomers generally had lower blood depuration 
      half-lives than the corresponding linear isomer. The most remarkable exception 
      was for the PFOS isomer containing an alpha-perfluoromethyl branch (1m-PFOS), 
      which was threefold more persistent than linear PFOS, possibly due to steric 
      shielding of the hydrophilic sulfonate moiety. For perfluoromonomethyl-branched 
      isomers of PFOS, a structure-property relationship was observed whereby branching 
      toward the sulfonate end of the perfluoroalkyl chain resulted in increased 
      half-lives. For PFHxS, PFOA, and PFOS, preferential elimination of branched 
      isomers occurred primarily via urine, whereas for PFNA preferential elimination 
      of the isopropyl isomer occurred via both urine and feces. Changes in the blood 
      isomer profiles over time and their inverse correlation to isomer elimination 
      patterns in urine, feces, or both provided unequivocal evidence of significant 
      isomer-specific biological handling. Source assignment based on PFA isomer 
      profiles in biota must therefore be conducted with caution, because isomer 
      profiles are unlikely to be conserved in biological samples.
FAU - Benskin, Jonathan P
AU  - Benskin JP
AD  - Division of Analytical and Environmental Toxicology, Department of Laboratory 
      Medicine and Pathology, University of Alberta, Edmonton, Alverta, Canada.
FAU - De Silva, Amila O
AU  - De Silva AO
FAU - Martin, Leah J
AU  - Martin LJ
FAU - Arsenault, Gilles
AU  - Arsenault G
FAU - McCrindle, Robert
AU  - McCrindle R
FAU - Riddell, Nicole
AU  - Riddell N
FAU - Mabury, Scott A
AU  - Mabury SA
FAU - Martin, Jonathan W
AU  - Martin JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081021
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Environmental Pollutants)
RN  - 0 (Hydrocarbons, Fluorinated)
SB  - IM
MH  - Animals
MH  - Environmental Pollutants/*administration & dosage/blood/*pharmacokinetics/urine
MH  - Feces/chemistry
MH  - Hydrocarbons, Fluorinated/*administration & dosage/blood/*pharmacokinetics/urine
MH  - Isomerism
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tissue Distribution
EDAT- 2008/10/23 09:00
MHDA- 2009/06/11 09:00
CRDT- 2008/10/23 09:00
PHST- 2008/05/21 00:00 [received]
PHST- 2008/08/08 00:00 [accepted]
PHST- 2008/10/23 09:00 [pubmed]
PHST- 2009/06/11 09:00 [medline]
PHST- 2008/10/23 09:00 [entrez]
AID - 08-239 [pii]
AID - 10.1897/08-239.1 [doi]
PST - ppublish
SO  - Environ Toxicol Chem. 2009 Mar;28(3):542-54. doi: 10.1897/08-239.1. Epub 2008 Oct 
      21.