PMID- 18940469
OWN - NLM
STAT- MEDLINE
DCOM- 20081104
LR  - 20081022
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 186
IP  - 2
DP  - 2008 Oct 15
TI  - Automated four-color interphase fluorescence in situ hybridization approach for 
      the simultaneous detection of specific aneuploidies of diagnostic and prognostic 
      significance in high hyperdiploid acute lymphoblastic leukemia.
PG  - 69-77
LID - 10.1016/j.cancergencyto.2008.06.008 [doi]
AB  - In high hyperdiploid acute lymphoblastic leukemia (ALL), the concurrence of 
      specific trisomies confers a more favorable outcome than hyperdiploidy alone. 
      Interphase fluorescence in situ hybridization (FISH) complements conventional 
      cytogenetics (CC) through its sensitivity and ability to detect chromosome 
      aberrations in nondividing cells. To overcome the limits of manual I-FISH, we 
      developed an automated four-color I-FISH approach and assessed its ability to 
      detect concurrent aneuploidies in ALL. I-FISH was performed using centromeric 
      probes for chromosomes 4, 6, 10, and 17. Parameters established for nucleus 
      selection and signal detection were evaluated. Cutoff values were determined. 
      Combinations of aneuploidies were considered relevant when each aneuploidy was 
      individually significant. Results obtained in 10 patient samples were compared 
      with those obtained with CC. Various combinations of aneuploidies were 
      identified. All clones detected by CC were observed also by I-FISH, and I-FISH 
      revealed numerous additional abnormal clones in all patients, ranging from < or 
      =1% to 31.6% of cells analyzed. We conclude that four-color automated I-FISH 
      permits the identification of concurrent aneuploidies of potential prognostic 
      significance. Large numbers of cells can be analyzed rapidly. The large number of 
      nuclei scored revealed a high level of chromosome variability both at diagnosis 
      and relapse, the prognostic significance of which is of considerable clinical 
      interest and merits further evaluation.
FAU - Blandin, Anna Talamo
AU  - Blandin AT
AD  - Cancer Cytogenetics Unit, Medical Genetics Service, University Hospital and 
      University of Lausanne (CHUV-UNIL), Lausanne, Switzerland.
FAU - Muhlematter, Dominique
AU  - Muhlematter D
FAU - Bougeon, Sandrine
AU  - Bougeon S
FAU - Gogniat, Celine
AU  - Gogniat C
FAU - Porter, Sarah
AU  - Porter S
FAU - Beyer, Valerie
AU  - Beyer V
FAU - Parlier, Valerie
AU  - Parlier V
FAU - Beckmann, Jacques S
AU  - Beckmann JS
FAU - van Melle, Guy
AU  - van Melle G
FAU - Jotterand, Martine
AU  - Jotterand M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Aneuploidy
MH  - Child, Preschool
MH  - Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Prognosis
MH  - Sensitivity and Specificity
MH  - Trisomy
EDAT- 2008/10/23 09:00
MHDA- 2008/11/05 09:00
CRDT- 2008/10/23 09:00
PHST- 2008/04/14 00:00 [received]
PHST- 2008/06/12 00:00 [revised]
PHST- 2008/06/16 00:00 [accepted]
PHST- 2008/10/23 09:00 [pubmed]
PHST- 2008/11/05 09:00 [medline]
PHST- 2008/10/23 09:00 [entrez]
AID - S0165-4608(08)00396-8 [pii]
AID - 10.1016/j.cancergencyto.2008.06.008 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2008 Oct 15;186(2):69-77. doi: 
      10.1016/j.cancergencyto.2008.06.008.