PMID- 18941796
OWN - NLM
STAT- MEDLINE
DCOM- 20090810
LR  - 20090520
IS  - 1615-7605 (Electronic)
IS  - 1615-7591 (Linking)
VI  - 32
IP  - 4
DP  - 2009 Jun
TI  - Electrochemical nitrite biosensor based on the immobilization of hemoglobin on an 
      electrode modified by multiwall carbon nanotubes and positively charged gold 
      nanoparticle.
PG  - 485-92
LID - 10.1007/s00449-008-0268-7 [doi]
AB  - The report is on an electrochemical biosensor with remarkably improved 
      sensitivity toward nitrite. In this strategy, positively charged gold 
      nanoparticle (PCNA) is used in combination with multiwall carbon nanotubes 
      (MWCNT) by electrostatic adsorption for fabricating PCNA/MWCNT films. Then 
      hemoglobin (Hb) biocatalyst will easily be attached to the surface of the 
      combination films aforementioned. After that, the Hb/PCNA films are immobilized 
      onto the Hb/PCNA/MWCNT films through layer-by-layer assembly technique. The 
      (Hb/PCNA)(2)/MWNT/GC electrode thus prepared exhibits enhanced electrocatalytic 
      behavior to the reduction of nitrite at -0.10 V versus SCE in 0.05 M H(2)SO(4) 
      solution. On condition of the low detecting potential and low pH, interference 
      caused by direct electrochemical oxidation or oxidizable substances can be 
      prevented. Therefore, the modified electrode shows fast response time, very high 
      sensitivity, good selectivity and stability. The current response of the sensor 
      increases linearly with nitrite concentration from a range of 3.6 x 10(-6) to 3.0 
      x 10(-3) M with a detection limit(S /N = 3) of 9.6 x 10(-7) M.
FAU - Zhang, Lingyan
AU  - Zhang L
AD  - Forensic Identification Center, College of Criminal Investigation, Southwest 
      University of Political Science and Law, 401120, Chongqing, China. zly100@126.com
FAU - Yi, Min
AU  - Yi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081022
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
RN  - 0 (Hemoglobins)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Nitrites)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - *Biosensing Techniques/standards/statistics & numerical data
MH  - Electrochemical Techniques/instrumentation/statistics & numerical data
MH  - Electrodes
MH  - Gold
MH  - Hemoglobins
MH  - Metal Nanoparticles/chemistry/ultrastructure
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Nanotubes, Carbon/chemistry/ultrastructure
MH  - Nitrites/*analysis/standards
MH  - Reference Standards
MH  - Sensitivity and Specificity
EDAT- 2008/10/23 09:00
MHDA- 2009/08/11 09:00
CRDT- 2008/10/23 09:00
PHST- 2008/02/04 00:00 [received]
PHST- 2008/09/26 00:00 [accepted]
PHST- 2008/10/23 09:00 [pubmed]
PHST- 2009/08/11 09:00 [medline]
PHST- 2008/10/23 09:00 [entrez]
AID - 10.1007/s00449-008-0268-7 [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2009 Jun;32(4):485-92. doi: 10.1007/s00449-008-0268-7. 
      Epub 2008 Oct 22.