PMID- 18941901
OWN - NLM
STAT- MEDLINE
DCOM- 20090204
LR  - 20230425
IS  - 1559-7113 (Electronic)
IS  - 1559-7105 (Print)
IS  - 1559-7105 (Linking)
VI  - 36
IP  - 1-4
DP  - 2008 Aug
TI  - Reporting neural activity with genetically encoded calcium indicators.
PG  - 69-86
LID - 10.1007/s11068-008-9029-4 [doi]
AB  - Genetically encoded calcium indicators (GECIs), based on recombinant fluorescent 
      proteins, have been engineered to observe calcium transients in living cells and 
      organisms. Through observation of calcium, these indicators also report neural 
      activity. We review progress in GECI construction and application, particularly 
      toward in vivo monitoring of sparse action potentials (APs). We summarize the 
      extrinsic and intrinsic factors that influence GECI performance. A simple model 
      of GECI response to AP firing demonstrates the relative significance of these 
      factors. We recommend a standardized protocol for evaluating GECIs in a 
      physiologically relevant context. A potential method of simultaneous optical 
      control and recording of neuronal circuits is presented.
FAU - Hires, S Andrew
AU  - Hires SA
AD  - Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Dr, 
      Ashburn, VA 20147, USA. hiresa@janelia.hhmi.org
FAU - Tian, Lin
AU  - Tian L
FAU - Looger, Loren L
AU  - Looger LL
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20081022
PL  - United States
TA  - Brain Cell Biol
JT  - Brain cell biology
JID - 101272887
RN  - 0 (Luminescent Proteins)
SB  - IM
MH  - Action Potentials/*physiology
MH  - Animals
MH  - Calcium Signaling/*physiology
MH  - Electrophysiology/methods
MH  - Luminescent Proteins/genetics/*metabolism
MH  - Microscopy, Confocal/methods
MH  - Neurons/metabolism/*physiology
MH  - Signal Transduction/*physiology
PMC - PMC2755531
MID - NIHMS143611
OID - NLM: HHMIMS143611
EDAT- 2008/10/23 09:00
MHDA- 2009/02/05 09:00
PMCR- 2009/10/02
CRDT- 2008/10/23 09:00
PHST- 2008/05/06 00:00 [received]
PHST- 2008/08/06 00:00 [accepted]
PHST- 2008/06/22 00:00 [revised]
PHST- 2008/10/23 09:00 [pubmed]
PHST- 2009/02/05 09:00 [medline]
PHST- 2008/10/23 09:00 [entrez]
PHST- 2009/10/02 00:00 [pmc-release]
AID - 10.1007/s11068-008-9029-4 [doi]
PST - ppublish
SO  - Brain Cell Biol. 2008 Aug;36(1-4):69-86. doi: 10.1007/s11068-008-9029-4. Epub 
      2008 Oct 22.