PMID- 18942710
OWN - NLM
STAT- MEDLINE
DCOM- 20090115
LR  - 20210105
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Linking)
VI  - 124
IP  - 2
DP  - 2009 Jan 15
TI  - Reciprocal activating interaction between 6-sulfo LacNAc+ dendritic cells and NK 
      cells.
PG  - 358-66
LID - 10.1002/ijc.23962 [doi]
AB  - Dendritic cells (DCs) display an extraordinary capacity to induce T-cell 
      responses providing the opportunity of DC-based cancer vaccination strategies. 
      Additional findings indicate that DCs may also play a crucial role for the 
      activation of natural killer (NK) cells, which are important effectors in innate 
      antitumor immunity. However, studies investigating the interaction between native 
      human DCs and NK cells are limited. Recently, we defined 6-sulfo LacNAc (slan) 
      DCs as a major subpopulation of myeloid human blood DCs, which represent 
      principal producers of the proinflammatory cytokines tumor necrosis factor-alpha 
      and interleukin (IL)-12. Functional data revealed that slanDCs efficiently induce 
      neoantigen-specific CD4+ T cells and activate tumor-reactive cytotoxic T cells. 
      When evaluating the crosstalk between slanDCs and NK cells in this study, we 
      found that lipopolysaccharide (LPS)-activated slanDCs efficiently enhance NK cell 
      CD69 expression and interferon (IFN)-gamma secretion. NK cell-mediated 
      tumor-directed cytotoxicity was significantly improved by slanDCs. NK cell 
      activation induced by slanDCs was critically dependent on IL-12. When 
      investigating the impact of NK cells on the immunostimulatory capacity of 
      slanDCs, we observed that they promote DC maturation. In addition, NK cells 
      strongly enhanced the secretion of immunomodulatory IL-12 and reduced the release 
      of immunosuppressive IL-10 by slanDCs. IFN-gamma and cell-to-cell contact 
      contributed to these effects. Furthermore, data revealed that DC-NK cell 
      crosstalk improves slanDC-mediated differentiation of naive CD4+ T lymphocytes 
      into IFN-gamma-producing Th1 cells. In conclusion, we demonstrate a reciprocal 
      activating interaction between slanDCs and NK cells, which may play a pivotal 
      role in the regulation of antitumor immunity.
CI  - Copyright (c) 2008 Wiley-Liss, Inc.
FAU - Wehner, Rebekka
AU  - Wehner R
AD  - Institute of Immunology, Department of Medicine I, Medical Faculty, Technical 
      University of Dresden, Dresden, Germany.
FAU - Lobel, Barbel
AU  - Lobel B
FAU - Bornhauser, Martin
AU  - Bornhauser M
FAU - Schakel, Knut
AU  - Schakel K
FAU - Cartellieri, Marc
AU  - Cartellieri M
FAU - Bachmann, Michael
AU  - Bachmann M
FAU - Rieber, Ernst Peter
AU  - Rieber EP
FAU - Schmitz, Marc
AU  - Schmitz M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (6-sulfo-LacNac)
RN  - 0 (Amino Sugars)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD69 antigen)
RN  - 0 (Cytokines)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Sugars/*metabolism
MH  - Antigens, CD/biosynthesis
MH  - Antigens, Differentiation, T-Lymphocyte/biosynthesis
MH  - Cytokines/metabolism
MH  - Dendritic Cells/cytology/*metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - K562 Cells
MH  - Killer Cells, Natural/*metabolism
MH  - Lectins, C-Type
MH  - Leukocytes, Mononuclear/cytology
MH  - Lipopolysaccharides/metabolism
MH  - Lymphocyte Activation
MH  - Models, Biological
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2008/10/24 09:00
MHDA- 2009/01/16 09:00
CRDT- 2008/10/24 09:00
PHST- 2008/10/24 09:00 [entrez]
PHST- 2008/10/24 09:00 [pubmed]
PHST- 2009/01/16 09:00 [medline]
AID - 10.1002/ijc.23962 [doi]
PST - ppublish
SO  - Int J Cancer. 2009 Jan 15;124(2):358-66. doi: 10.1002/ijc.23962.