PMID- 18943042
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20081023
IS  - 0031-949X (Print)
IS  - 0031-949X (Linking)
VI  - 95
IP  - 4
DP  - 2005 Apr
TI  - Changes in Protein Kinase A Activity Accompany Sclerotial Development in 
      Sclerotinia sclerotiorum.
PG  - 397-404
LID - 10.1094/PHYTO-95-0397 [doi]
AB  - ABSTRACT Sclerotia of Sclerotinia sclerotiorum are pigmented, multihyphal 
      structures that play a central role in the life and infection cycles of this 
      pathogen. Sclerotial formation has been shown to be affected by increased 
      intracellular cAMP levels. Cyclic AMP (cAMP) is a key modulator of cAMP-dependent 
      protein kinase A (PKA) and the latter may prove to play a significant role in 
      sclerotial development. Therefore, we monitored changes in relative PKA activity 
      levels during sclerotial development. To do so, we first developed conditions for 
      near-synchronous sclerotial development in culture, based on hyphal maceration 
      and filtering. Relative PKA activity levels increased during the white-sclerotium 
      stage in the wild-type strain, while low levels were maintained in 
      nonsclerotium-producing mutants. Furthermore, applying caffeine, an inducer of 
      PKA activity, resulted in increased relative PKA activity levels and was 
      correlated with the formation of sclerotial initial-like aggregates in cultures 
      of the non-sclerotium-producing mutants. In addition, low PKA activities were 
      found in an antisense smk1 strain, which exhibits low 
      extracellular-signal-regulated kinase (ERK)-type mitogen-activated protein kinase 
      (MAPK) activity, and does not produce sclerotia. The changes in PKA activity, as 
      well as the abundance of phosphorylated MAPKs (ERK-like as well as p38-like) that 
      accompany sclerotial development in a distinct developmental phase manner 
      represent a potential target for antifungal intervention.
FAU - Harel, A
AU  - Harel A
FAU - Gorovits, R
AU  - Gorovits R
FAU - Yarden, O
AU  - Yarden O
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Phytopathology
JT  - Phytopathology
JID - 9427222
EDAT- 2008/10/24 09:00
MHDA- 2008/10/24 09:01
CRDT- 2008/10/24 09:00
PHST- 2008/10/24 09:00 [pubmed]
PHST- 2008/10/24 09:01 [medline]
PHST- 2008/10/24 09:00 [entrez]
AID - 10.1094/PHYTO-95-0397 [doi]
PST - ppublish
SO  - Phytopathology. 2005 Apr;95(4):397-404. doi: 10.1094/PHYTO-95-0397.