PMID- 18946350
OWN - NLM
STAT- MEDLINE
DCOM- 20081113
LR  - 20220321
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 86
IP  - 8
DP  - 2008 Oct 27
TI  - "Natural" human leukocyte antigen antibodies found in nonalloimmunized healthy 
      males.
PG  - 1111-5
LID - 10.1097/TP.0b013e318186d87b [doi]
AB  - BACKGROUND: Human leukocyte antigen (HLA) antibodies were normally not found in 
      subjects who have not been immunized by pregnancies, transfusions, or 
      transplants. But with new methodology, we now see that HLA antibodies are often 
      found in nonalloimmunized males. METHODS: The sera of 424 healthy male donors 
      were tested with single antigen Luminex beads. RESULTS: Human leukocyte antigen 
      antibodies were detected in 63% of 424 male blood donors when a fluorescent value 
      of more than 1000 was used as the cutoff. Antibodies to class I was found in 42%, 
      class II in 11% and both in 12%. Five males who were tested eight times over a 
      6-month period consistently had the same specificity at similar strength levels 
      at each testing. The antibodies reacted with specificities that are rare in the 
      general population: 18.9% had antibodies to A*3002; more than 10% had antibodies 
      to A*3101, B76, B*8201, and Cw*1701. About half of the donors with antibodies had 
      one or two specificities; the other half had three or more specificities. Among 
      those with class II specificities, 20.5% had antibodies to DPA1*0201/DPB1*0101, 
      and 10.8% to DQA1*0503/DQB1*0301. Because the above data were obtained by testing 
      sera of 424 Mexican donors, as a check, 29 males in Los Angeles were tested and 
      shown to have similar specificities at roughly similar frequencies. CONCLUSIONS: 
      Normal males were found to have HLA antibodies to infrequent HLA specificities. 
      It is likely that these HLA antibodies are produced to cross-reactive epitopes 
      found in microorganisms, ingested proteins and allergens-making them natural 
      antibodies.
FAU - Morales-Buenrostro, Luis E
AU  - Morales-Buenrostro LE
AD  - Department of Nephrology and Mineral Metabolism, National Institute of Medical 
      Sciences and Nutrition Salvador Zubiran, Mexico City, Mexico.
FAU - Terasaki, Paul I
AU  - Terasaki PI
FAU - Marino-Vazquez, Lluvia A
AU  - Marino-Vazquez LA
FAU - Lee, Jar-How
AU  - Lee JH
FAU - El-Awar, Nadim
AU  - El-Awar N
FAU - Alberu, Josefina
AU  - Alberu J
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antibodies)
RN  - 0 (Autoantibodies)
RN  - 0 (HLA Antigens)
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - Adult
MH  - Antibodies/*blood
MH  - Antibody Specificity
MH  - Autoantibodies/blood
MH  - Cell Line
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - Immunosorbent Techniques
MH  - Los Angeles
MH  - Male
MH  - Mexico
MH  - Reagent Kits, Diagnostic
MH  - Reproducibility of Results
MH  - Time Factors
EDAT- 2008/10/24 09:00
MHDA- 2008/11/14 09:00
CRDT- 2008/10/24 09:00
PHST- 2008/10/24 09:00 [pubmed]
PHST- 2008/11/14 09:00 [medline]
PHST- 2008/10/24 09:00 [entrez]
AID - 00007890-200810270-00015 [pii]
AID - 10.1097/TP.0b013e318186d87b [doi]
PST - ppublish
SO  - Transplantation. 2008 Oct 27;86(8):1111-5. doi: 10.1097/TP.0b013e318186d87b.