PMID- 18946734
OWN - NLM
STAT- MEDLINE
DCOM- 20090219
LR  - 20181113
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 33
IP  - 12
DP  - 2008 Dec
TI  - Localization of abasic sites and single-strand breaks in mitochondrial DNA from 
      brain of aged rat, treated or not with caloric restriction diet.
PG  - 2609-14
LID - 10.1007/s11064-008-9866-7 [doi]
AB  - According to the "mitochondrial theory of aging" the lifelong accumulation of 
      various kinds of damage to mitochondrial DNA (mtDNA) has been related to the 
      age-dependent mitochondrial bioenergetic dysfunction. Caloric restriction (CR) 
      diet is able to prevent or delay the onset of several age-related damages to 
      mtDNA. The effects of aging and CR on the presence of abasic sites and 
      single-strand breaks of the sugar-phosphate backbone in mtDNA have been analyzed 
      by applying Ligation Mediated-PCR to a H strand region of brain mtDNA from young 
      and old ad libitum-fed and old CR-treated rats. The region, encompassing the 
      Direct Repeat 1 of the 4,834 bp-long deletion, is highly damaged in the old ad 
      libitum-fed animals with respect to the young ones, whereas in the CR rats it 
      shows a much lower extent of damage. The data confirm, at single nucleotide 
      resolution, the protective effect of CR on the age-related mtDNA damage.
FAU - Lezza, A M S
AU  - Lezza AM
AD  - Department of Biochemistry and Molecular Biology Ernesto Quagliariello, 
      University of Bari, Via Orabona, 4, 70125, Bari, Italy.
FAU - Fallacara, F P
AU  - Fallacara FP
FAU - Pesce, V
AU  - Pesce V
FAU - Leeuwenburgh, C
AU  - Leeuwenburgh C
FAU - Cantatore, P
AU  - Cantatore P
FAU - Gadaleta, M N
AU  - Gadaleta MN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081023
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Single-Stranded)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Autoradiography
MH  - Base Sequence
MH  - Brain/*metabolism
MH  - *Caloric Restriction
MH  - *DNA Damage
MH  - DNA Primers
MH  - DNA, Single-Stranded/*metabolism
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Rats, Inbred F344
EDAT- 2008/10/24 09:00
MHDA- 2009/02/20 09:00
CRDT- 2008/10/24 09:00
PHST- 2008/05/30 00:00 [received]
PHST- 2008/10/03 00:00 [accepted]
PHST- 2008/10/24 09:00 [pubmed]
PHST- 2009/02/20 09:00 [medline]
PHST- 2008/10/24 09:00 [entrez]
AID - 10.1007/s11064-008-9866-7 [doi]
PST - ppublish
SO  - Neurochem Res. 2008 Dec;33(12):2609-14. doi: 10.1007/s11064-008-9866-7. Epub 2008 
      Oct 23.