PMID- 18947863
OWN - NLM
STAT- MEDLINE
DCOM- 20090127
LR  - 20210409
IS  - 1097-6825 (Electronic)
IS  - 0091-6749 (Linking)
VI  - 123
IP  - 1
DP  - 2009 Jan
TI  - Allergen-enhanced thrombomodulin (blood dendritic cell antigen 3, CD141) 
      expression on dendritic cells is associated with a TH2-skewed immune response.
PG  - 209-216.e4
LID - 10.1016/j.jaci.2008.09.009 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are important in allergic diseases such as 
      asthma, although little is known regarding the mechanisms by which DCs induce 
      T(H)2-polarized responses in atopic individuals. It has been suggested that 
      intrinsic properties of allergens can directly stimulate T(H)2 polarizing 
      functions of DCs, but little is known of the underlying mechanisms. OBJECTIVE: To 
      identify novel genes expressed by house dust mite (HDM) allergen-exposed DCs. 
      METHODS: We screened for allergen-induced gene expression by microarray, and 
      validated differentially expressed genes at the mRNA and protein levels. RESULTS: 
      Thrombomodulin (CD141, blood dendritic cell antigen 3) expression by microarray 
      was higher on HDM-stimulated DCs from atopic (relative to nonatopic) individuals. 
      These findings were confirmed at both the mRNA and protein levels in an 
      independent group. Purified thrombomodulin(+) DCs induced a strongly 
      T(H)2-polarized cytokine response by allergen-specific T cells compared with DCs 
      lacking thrombomodulin. In vivo, thrombomodulin(+) circulating DCs were 
      significantly more frequent in subjects with HDM allergy and asthma, compared 
      with control subjects. Furthermore, thrombomodulin expression in blood leukocytes 
      was higher in children with acute asthma than at convalescence 6 weeks later. 
      CONCLUSION: Thrombomodulin expression on DCs may be involved in the pathogenesis 
      of atopy and asthma.
FAU - Yerkovich, Stephanie T
AU  - Yerkovich ST
AD  - Telethon Institute for Child Health Research, Centre for Child Health Research, 
      Faculty of Medicine and Dentistry, University of Western Australia, Perth, 
      Australia.
FAU - Roponen, Marjut
AU  - Roponen M
FAU - Smith, Miranda E
AU  - Smith ME
FAU - McKenna, Kathy
AU  - McKenna K
FAU - Bosco, Anthony
AU  - Bosco A
FAU - Subrata, Lily S
AU  - Subrata LS
FAU - Mamessier, Emilie
AU  - Mamessier E
FAU - Wikstrom, Matthew E
AU  - Wikstrom ME
FAU - Le Souef, Peter
AU  - Le Souef P
FAU - Sly, Peter D
AU  - Sly PD
FAU - Holt, Patrick G
AU  - Holt PG
FAU - Upham, John W
AU  - Upham JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081022
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Antigens, Surface)
RN  - 0 (THBD protein, human)
RN  - 0 (Thrombomodulin)
SB  - IM
CIN - J Allergy Clin Immunol. 2009 May;123(5):1192; author reply 1192-3. PMID: 19251314
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antigens, Dermatophagoides/*immunology/pharmacology
MH  - Antigens, Surface/biosynthesis/*immunology
MH  - Asthma/*immunology/metabolism/pathology
MH  - Child
MH  - Child, Preschool
MH  - Convalescence
MH  - Dendritic Cells/*immunology/metabolism/*pathology
MH  - Female
MH  - Gene Expression Profiling/methods
MH  - Gene Expression Regulation/drug effects/immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis/methods
MH  - Th2 Cells/*immunology/pathology
MH  - Thrombomodulin/biosynthesis/*immunology
EDAT- 2008/10/25 09:00
MHDA- 2009/01/28 09:00
CRDT- 2008/10/25 09:00
PHST- 2008/01/30 00:00 [received]
PHST- 2008/09/03 00:00 [revised]
PHST- 2008/09/05 00:00 [accepted]
PHST- 2008/10/25 09:00 [pubmed]
PHST- 2009/01/28 09:00 [medline]
PHST- 2008/10/25 09:00 [entrez]
AID - S0091-6749(08)01670-9 [pii]
AID - 10.1016/j.jaci.2008.09.009 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2009 Jan;123(1):209-216.e4. doi: 
      10.1016/j.jaci.2008.09.009. Epub 2008 Oct 22.