PMID- 18948208
OWN - NLM
STAT- MEDLINE
DCOM- 20090302
LR  - 20090210
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 26
IP  - 1
DP  - 2009 Jan
TI  - Identification of two differentially expressed forms of progranulin mRNA in the 
      teleost fish Paralichthys olivaceus.
PG  - 177-82
LID - 10.1016/j.fsi.2008.09.009 [doi]
AB  - Two forms of progranulin mRNA were isolated from kidney and spleen cDNA libraries 
      of the olive flounder, Paralichthys olivaceus. The 3'-untranslated regions 
      (3'-UTRs) of these flounder progranulin (f-pgrn) mRNAs differed in a 
      20-nucleotide sequence element (5'-AACTGATTACGTTCAACAAC-3') that was present in 
      one mRNA (designated f-pgrn type II) and not in the other (designated f-pgrn type 
      I). Both mRNA sequences contained an open reading frame encoding a 289-amino-acid 
      polypeptide of approximately 33 kDa. Southern blot analysis of the P. olivaceus 
      flounder genome using an f-pgrn cDNA probe and a PCR-based approach identified a 
      single copy of f-pgrn corresponding to the type II mRNA. The expression profiles 
      of the two types of f-pgrn mRNA differed from each other and were tissue- and 
      condition-dependent. The type II mRNA was detected abundantly in studied tissues 
      (gill, kidney, spleen, and intestine) of non-stimulated healthy flounders. The 
      type I mRNA was rarely expressed in any tissues of healthy flounders, but it was 
      continuously increased in the examined tissues of flounders after the 
      intraperitoneal injection of lipopolysaccharide. On the other hand, the 
      expression of type II mRNA was decreased in inverse proportion to the type I mRNA 
      in the LSP-stimulated flounders. These results suggest that type I and type II 
      f-pgrn mRNA are translated into different proteins with different activities in 
      the immune system of flounder.
FAU - Nam, Bo-Hye
AU  - Nam BH
AD  - Biotechnology Research Institute, National Fisheries Research and Development 
      Institute, Gijang-gun, Busan, Republic of Korea. bhnam@hanmail.net
FAU - Park, Eun-Mi
AU  - Park EM
FAU - Kim, Young-Ok
AU  - Kim YO
FAU - Kong, Hee Jeong
AU  - Kong HJ
FAU - Kim, Woo-Jin
AU  - Kim WJ
FAU - Kang, Jung-Ha
AU  - Kang JH
FAU - Ji, Young-Ju
AU  - Ji YJ
FAU - Lee, Sang-Jun
AU  - Lee SJ
FAU - Choi, Tae-Jin
AU  - Choi TJ
LA  - eng
SI  - GENBANK/EU419019
SI  - GENBANK/EU419020
SI  - GENBANK/EU419021
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081001
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Flounder/*genetics/*immunology
MH  - Gene Expression Regulation/drug effects
MH  - Gene Order
MH  - Intercellular Signaling Peptides and Proteins/chemistry/*genetics
MH  - Lipopolysaccharides/pharmacology
MH  - Molecular Sequence Data
MH  - RNA, Messenger/immunology
MH  - Sequence Alignment
EDAT- 2008/10/25 09:00
MHDA- 2009/03/03 09:00
CRDT- 2008/10/25 09:00
PHST- 2008/04/25 00:00 [received]
PHST- 2008/09/01 00:00 [revised]
PHST- 2008/09/18 00:00 [accepted]
PHST- 2008/10/25 09:00 [pubmed]
PHST- 2009/03/03 09:00 [medline]
PHST- 2008/10/25 09:00 [entrez]
AID - S1050-4648(08)00219-2 [pii]
AID - 10.1016/j.fsi.2008.09.009 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2009 Jan;26(1):177-82. doi: 10.1016/j.fsi.2008.09.009. 
      Epub 2008 Oct 1.