PMID- 18950847
OWN - NLM
STAT- MEDLINE
DCOM- 20090528
LR  - 20090126
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 71
IP  - 4
DP  - 2009 Mar 1
TI  - MALDI-TOF "fingerprint" phospholipid mass spectra allow the differentiation 
      between ruminantia and feloideae spermatozoa.
PG  - 568-75
LID - 10.1016/j.theriogenology.2008.08.023 [doi]
AB  - The detailed comparative analysis of sperm lipids could essentially contribute to 
      a better understanding of membrane function in the context of fertilization and, 
      moreover, of sperm preservation. The application of sensitive analytical methods 
      is particularly necessary for endangered species as the available amount of 
      spermatozoa (and, accordingly, extractable lipids) is strongly limited. It will 
      be shown that matrix-assisted laser desorption and ionization time-of-flight 
      (MALDI-TOF) mass spectrometry (MS) is a fast, simple and sensitive method for the 
      determination of the phospholipid composition of spermatozoa from several 
      ruminantia (cattle, roe deer, Klipspringer) and feloideae species (domestic cat, 
      Siberian tiger, fosa). Characteristic "fingerprints" are obtained from the 
      positive ion spectra that allow the differentiation between both animal groups. 
      In contrast to the lipid extracts of ruminantia spermatozoa which predominantly 
      contain ether lipids including essential amounts of plasmalogens, the more 
      complex phospholipid composition of feloideae spermatozoa is clearly dominated by 
      diacyl phospholipids and contains only marginal amounts of plasmalogens. It will 
      also be shown that the lipid compositions of ejaculated, electroejaculated and 
      cauda epididymal spermatozoa of the same species are very similar and give 
      comparable data. Therefore, the analysis of ejaculated spermatozoa is not an 
      absolute must.
FAU - Fuchs, B
AU  - Fuchs B
AD  - University of Leipzig, Medical Faculty, Institute of Medical Physics and 
      Biophysics, Leipzig, Germany.
FAU - Jakop, U
AU  - Jakop U
FAU - Goritz, F
AU  - Goritz F
FAU - Hermes, R
AU  - Hermes R
FAU - Hildebrandt, T
AU  - Hildebrandt T
FAU - Schiller, J
AU  - Schiller J
FAU - Muller, K
AU  - Muller K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081023
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Phospholipids)
SB  - IM
MH  - Animals
MH  - Felidae/*physiology
MH  - Male
MH  - Phospholipids/*chemistry/metabolism
MH  - Ruminants/*physiology
MH  - Species Specificity
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*veterinary
MH  - Spermatozoa/*cytology/physiology
EDAT- 2008/10/28 09:00
MHDA- 2009/05/29 09:00
CRDT- 2008/10/28 09:00
PHST- 2008/04/24 00:00 [received]
PHST- 2008/07/03 00:00 [revised]
PHST- 2008/08/17 00:00 [accepted]
PHST- 2008/10/28 09:00 [pubmed]
PHST- 2009/05/29 09:00 [medline]
PHST- 2008/10/28 09:00 [entrez]
AID - S0093-691X(08)00618-3 [pii]
AID - 10.1016/j.theriogenology.2008.08.023 [doi]
PST - ppublish
SO  - Theriogenology. 2009 Mar 1;71(4):568-75. doi: 
      10.1016/j.theriogenology.2008.08.023. Epub 2008 Oct 23.