PMID- 18951623
OWN - NLM
STAT- MEDLINE
DCOM- 20090327
LR  - 20081201
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 71
IP  - 1
DP  - 2009 Jan 1
TI  - Superovulation and embryo transfer in Holstein cattle using sexed sperm.
PG  - 68-73
LID - 10.1016/j.theriogenology.2008.09.016 [doi]
AB  - The use of sexed bull sperm in multiple ovulation and embryo transfer (MOET) 
      programs for Holsteins was evaluated for (1) heifers housed at a commercial 
      embryo transfer (ET) facility (Experiments 1 and 2), and (2) heifers and cows on 
      dairy farms (Experiment 3). In Experiment 1, superstimulated heifers were 
      inseminated with 5 x10(6) sexed (X-sorted; n=5) or unsexed (n=5) frozen-thawed 
      sperm from one bull at 12 and 24h after estrus detection. No difference was 
      observed in the rates of transferable embryos (53.4% vs 68.1%), degenerate 
      embryos (24.8% vs 26.6%) and unfertilized ova (21.8% vs 5.3%) between sexed and 
      unsexed sperm, respectively, except for the percent of female transferable 
      embryos diagnosed by embryo sexing (100% vs 49.3%, P<0.0001). In Experiment 2, 
      donors were inseminated twice with 5 x10(6) sexed unfrozen sperm (n=10) or sexed 
      frozen-thawed sperm (n=9). Embryo production rates for both treatments were 
      similar to that observed on a commercial ET facility using unsexed sperm. 
      Pregnancy rates for frozen-thawed embryos were similar for sexed and unsexed 
      sperm (70.4% vs 72.4%, respectively). In Experiment 3, 99 flushes were conducted 
      using sexed frozen-thawed sperm from nine bulls but an overall statistical 
      analysis was not completed because the use of bulls was not balanced. However, 
      for one bull with balanced usage, the rate of transferable embryos was higher in 
      heifers than in cows (P<0.05) inseminated twice with 5 x10(6) sperm/dose (10 
      x10(6) total). We concluded that the use of sexed frozen-thawed sperm (> or =90% 
      X-sperm biased and 10 x10(6) total sperm) may be economically viable for 
      commercial MOET programs in Holstein heifers.
FAU - Hayakawa, H
AU  - Hayakawa H
AD  - Genetics Hokkaido, Shimizu 089-0103, Japan. hayakawa@gh-assoc.ne.jp
FAU - Hirai, T
AU  - Hirai T
FAU - Takimoto, A
AU  - Takimoto A
FAU - Ideta, A
AU  - Ideta A
FAU - Aoyagi, Y
AU  - Aoyagi Y
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
DEP - 20081031
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
SB  - IM
MH  - Animals
MH  - Cattle/*physiology
MH  - Embryo Transfer/*veterinary
MH  - Female
MH  - Male
MH  - Semen Preservation/veterinary
MH  - Sex Preselection/*veterinary
MH  - Superovulation/*physiology
EDAT- 2008/10/28 09:00
MHDA- 2009/03/28 09:00
CRDT- 2008/10/28 09:00
PHST- 2008/10/28 09:00 [pubmed]
PHST- 2009/03/28 09:00 [medline]
PHST- 2008/10/28 09:00 [entrez]
AID - S0093-691X(08)00672-9 [pii]
AID - 10.1016/j.theriogenology.2008.09.016 [doi]
PST - ppublish
SO  - Theriogenology. 2009 Jan 1;71(1):68-73. doi: 
      10.1016/j.theriogenology.2008.09.016. Epub 2008 Oct 31.