PMID- 18951915
OWN - NLM
STAT- MEDLINE
DCOM- 20090312
LR  - 20200401
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Print)
IS  - 0145-305X (Linking)
VI  - 33
IP  - 4
DP  - 2009 Apr
TI  - Porcine DC-SIGN: molecular cloning, gene structure, tissue distribution and 
      binding characteristics.
PG  - 464-80
LID - 10.1016/j.dci.2008.09.010 [doi]
AB  - DC-SIGN, a human C-type lectin, is involved in the transmission of many enveloped 
      viruses. Here we report the cloning and characterization of the cDNA and gene 
      encoding porcine DC-SIGN (pDC-SIGN). The full-length pDC-SIGN cDNA encodes a type 
      II transmembrane protein of 240 amino acids. Phylogenetic analysis revealed that 
      pDC-SIGN, together with bovine, canis and equine DC-SIGN, are more closely 
      related to mouse SIGNR7 and SIGNR8 than to human DC-SIGN. pDC-SIGN has the same 
      gene structure as bovine, canis DC-SIGN and mouse SIGNR8 with eight exons. 
      pDC-SIGN mRNA expression was detected in pig spleen, thymus, lymph node, lung, 
      bone marrow and muscles. pDC-SIGN protein was found to express on the surface of 
      monocyte-derived macrophages and dendritic cells, alveolar macrophages, lymph 
      node sinusoidal macrophage-like, dendritic-like and endothelial cells but not of 
      monocytes, peripheral blood lymphocytes or lymph node lymphocytes. A BHK cell 
      line stably expressing pDC-SIGN binds to human ICAM-3 and ICAM-2 immunoadhesins 
      in a calcium-dependent manner, and enhances the transmission of porcine 
      reproductive and respiratory syndrome virus (PRRSV) to target cells in trans. The 
      results will help better understand the biological role(s) of DC-SIGN family in 
      innate immunity during the evolutionary process.
FAU - Huang, Y W
AU  - Huang YW
AD  - Center for Molecular Medicine and Infectious Diseases, Department of Biomedical 
      Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic 
      Institute and State University, 1410 Price's Fork Road, Blacksburg, VA 
      24061-0342, USA.
FAU - Dryman, B A
AU  - Dryman BA
FAU - Li, W
AU  - Li W
FAU - Meng, X J
AU  - Meng XJ
LA  - eng
SI  - GENBANK/EU684955
SI  - GENBANK/EU684956
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081023
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Antigens, CD)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (DNA, Complementary)
RN  - 0 (ICAM2 protein, human)
RN  - 0 (ICAM3 protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Base Sequence
MH  - Cattle
MH  - Cell Adhesion Molecules/classification/genetics/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Humans
MH  - Lectins, C-Type/classification/genetics/*metabolism
MH  - Liver/cytology/metabolism
MH  - Lymph Nodes/cytology/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Receptors, Cell Surface/classification/genetics/*metabolism
MH  - Sequence Alignment
MH  - Sus scrofa/genetics/*immunology
MH  - Tissue Distribution
PMC - PMC7103218
EDAT- 2008/10/28 09:00
MHDA- 2009/03/13 09:00
PMCR- 2008/10/23
CRDT- 2008/10/28 09:00
PHST- 2008/06/25 00:00 [received]
PHST- 2008/09/14 00:00 [revised]
PHST- 2008/09/16 00:00 [accepted]
PHST- 2008/10/28 09:00 [pubmed]
PHST- 2009/03/13 09:00 [medline]
PHST- 2008/10/28 09:00 [entrez]
PHST- 2008/10/23 00:00 [pmc-release]
AID - S0145-305X(08)00209-7 [pii]
AID - 10.1016/j.dci.2008.09.010 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2009 Apr;33(4):464-80. doi: 10.1016/j.dci.2008.09.010. Epub 
      2008 Oct 23.