PMID- 18951970
OWN - NLM
STAT- MEDLINE
DCOM- 20090415
LR  - 20191003
IS  - 1089-8646 (Electronic)
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 93
IP  - 4
DP  - 2009 Apr
TI  - A high-resolution cat radiation hybrid and integrated FISH mapping resource for 
      phylogenomic studies across Felidae.
PG  - 299-304
LID - 10.1016/j.ygeno.2008.09.010 [doi]
AB  - We describe the construction of a high-resolution radiation hybrid (RH) map of 
      the domestic cat genome, which includes 2662 markers, translating to an estimated 
      average intermarker distance of 939 kilobases (kb). Targeted marker selection 
      utilized the recent feline 1.9x genome assembly, concentrating on regions of low 
      marker density on feline autosomes and the X chromosome, in addition to regions 
      flanking interspecies chromosomal breakpoints. Average gap (breakpoint) size 
      between cat-human ordered conserved segments is less than 900 kb. The map was 
      used for a fine-scale comparison of conserved syntenic blocks with the human and 
      canine genomes. Corroborative fluorescence in situ hybridization (FISH) data were 
      generated using 129 domestic cat BAC clones as probes, providing independent 
      confirmation of the long-range correctness of the map. Cross-species 
      hybridization of BAC probes on divergent felids from the genera Profelis (serval) 
      and Panthera (snow leopard) provides further evidence for karyotypic conservation 
      within felids, and demonstrates the utility of such probes for future studies of 
      chromosome evolution within the cat family and in related carnivores. The 
      integrated map constitutes a comprehensive framework for identifying genes 
      controlling feline phenotypes of interest, and to aid in assembly of a higher 
      coverage feline genome sequence.
FAU - Davis, Brian W
AU  - Davis BW
AD  - Department of Veterinary Integrative Biosciences, College of Veterinary Medicine 
      and Biomedical Sciences, Texas A&M University, Mail Stop 4458, College Station, 
      TX 77843-4458, USA.
FAU - Raudsepp, Terje
AU  - Raudsepp T
FAU - Pearks Wilkerson, Alison J
AU  - Pearks Wilkerson AJ
FAU - Agarwala, Richa
AU  - Agarwala R
FAU - Schaffer, Alejandro A
AU  - Schaffer AA
FAU - Houck, Marlys
AU  - Houck M
FAU - Chowdhary, Bhanu P
AU  - Chowdhary BP
FAU - Murphy, William J
AU  - Murphy WJ
LA  - eng
GR  - Z01 LM000097-06/Intramural NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20081105
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Cats/*genetics
MH  - Felidae/*genetics
MH  - Genetic Markers
MH  - *Genome
MH  - Genotype
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Phylogeny
MH  - Radiation Hybrid Mapping/*methods
MH  - Synteny/genetics
PMC - PMC2656592
MID - NIHMS76344
EDAT- 2008/10/28 09:00
MHDA- 2009/04/16 09:00
PMCR- 2010/04/01
CRDT- 2008/10/28 09:00
PHST- 2008/08/05 00:00 [received]
PHST- 2008/09/21 00:00 [revised]
PHST- 2008/09/23 00:00 [accepted]
PHST- 2008/10/28 09:00 [pubmed]
PHST- 2009/04/16 09:00 [medline]
PHST- 2008/10/28 09:00 [entrez]
PHST- 2010/04/01 00:00 [pmc-release]
AID - S0888-7543(08)00230-9 [pii]
AID - 10.1016/j.ygeno.2008.09.010 [doi]
PST - ppublish
SO  - Genomics. 2009 Apr;93(4):299-304. doi: 10.1016/j.ygeno.2008.09.010. Epub 2008 Nov 
      5.