PMID- 18952234
OWN - NLM
STAT- MEDLINE
DCOM- 20090708
LR  - 20210103
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 154
IP  - 1
DP  - 2009 Jun 1
TI  - A comparison of monocyte counts and ex vivo and in vitro monocyte cytokine 
      production after major surgical trauma.
PG  - 91-8
LID - 10.1016/j.jss.2008.06.003 [doi]
AB  - BACKGROUND: Impaired function of cluster of differentiation 14-positive (CD14+) 
      monocytes (MOs) after major surgical trauma is believed to predispose to 
      infectious complications. Postoperative decreases in human leukocyte antigen 
      (HLA)-DR expression, tumor necrosis factor-alpha (TNF-alpha) production and 
      interleukin (IL)-12 synthesis have been reported. There are no studies comparing 
      absolute MO counts and MO cytokine synthesis in peripheral blood and stimulated 
      cultures. METHODS: The study group included 10 low-risk patients undergoing 
      elective cardiac surgery with extracorporeal circulation. Preoperatively (d0) and 
      on the first (d1) and third (d3) postoperative d, we analyzed leukocyte counts, 
      CD14+ MO absolute counts, HLA-DR expression, and stimulated IL-12 and TNF-alpha 
      synthesis using flow cytometry. In addition, IL-12 and TNF-alpha release in 
      stimulated whole blood cultures was assayed. RESULTS: Whereas the absolute 
      numbers of leukocytes and CD14+ MOs were significantly elevated, HLA-DR 
      expression was suppressed postoperatively. The proportion of TNF-alpha- and 
      IL-12-producing MOs was reduced after surgery. This, however, led to a 
      significant postoperative decrease only in the absolute numbers of peripheral 
      blood IL-12+ MOs. IL-12 secretion was postoperatively reduced in whole blood 
      cultures. The IL-12-synthesizing capacity of IL-12+ MOs was significantly reduced 
      only on d1. CONCLUSIONS: The immediate postoperative period is associated with an 
      increase in the absolute MO numbers and an impairment of MO function, which is 
      reflected in a reduced capacity to synthesize IL-12 and TNF-alpha and a decreased 
      ability to express HLA-DR and present antigens. Whereas the cytokine-producing 
      capacity returns to normal levels on d3, the suppression of HLA-DR expression 
      persists.
FAU - Franke, Axel
AU  - Franke A
AD  - Department of Trauma and Reconstructive Surgery, German Armed Forces Central 
      Hospital, Koblenz, Germany.
FAU - Lante, Wolfgang
AU  - Lante W
FAU - Kollig, Erwin
AU  - Kollig E
FAU - Markewitz, Andreas
AU  - Markewitz A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080711
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Cytokines)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Hemoglobins)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Aged
MH  - *Cardiopulmonary Bypass
MH  - Cell Culture Techniques
MH  - Cytokines/*biosynthesis
MH  - Elective Surgical Procedures
MH  - Female
MH  - Flow Cytometry
MH  - HLA-DR Antigens/analysis
MH  - Heart Valve Prosthesis Implantation
MH  - Hemoglobins/metabolism
MH  - Humans
MH  - Interleukin-12/biosynthesis
MH  - *Leukocyte Count
MH  - Lipopolysaccharide Receptors/blood
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Monocytes/cytology/drug effects/*physiology
MH  - Postoperative Period
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2008/10/28 09:00
MHDA- 2009/07/09 09:00
CRDT- 2008/10/28 09:00
PHST- 2008/02/26 00:00 [received]
PHST- 2008/04/30 00:00 [revised]
PHST- 2008/06/03 00:00 [accepted]
PHST- 2008/10/28 09:00 [pubmed]
PHST- 2009/07/09 09:00 [medline]
PHST- 2008/10/28 09:00 [entrez]
AID - S0022-4804(08)00397-1 [pii]
AID - 10.1016/j.jss.2008.06.003 [doi]
PST - ppublish
SO  - J Surg Res. 2009 Jun 1;154(1):91-8. doi: 10.1016/j.jss.2008.06.003. Epub 2008 Jul 
      11.